Restriction map for
pF1KE3557
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MfeI
MflI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1264 GACGTC GACGT^C
AccIII 521 TCCGGA T^CCGGA
AhdI 862 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 750 GATCC GGATC(4/5)
AocI 612 CCTGAGG CC^T[ACGT]AGG
Aor51HI 878 AGCGCT AGC^GCT
Asp700I 1290 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 862 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1104 GGTGCC G^G[CT][GA]CC
BbvCI 657 GCTGAGG CCTCAGC(-5/-2)
BsaI 1558 GAGACC GGTCTC(1/5)
BseAI 521 TCCGGA T^CCGGA
BsiEI 916 CGACCG CG[GA][CT]^CG
BsmBI 55 GAGACG CGTCTC(1/5)
BsmI 921 GAATGC GAATGC(1/-1)
BspEI 521 TCCGGA T^CCGGA
BsrBI 1421 CCGCTC CCGCTC(-3/-3)
BssHII 1067 GCGCGC G^CGCGC
BstAPI 1020 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 612 CCTGAGG CC^T[ACGT]AGG
Eam1105I 862 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 878 AGCGCT AGC^GCT
Eco81I 612 CCTGAGG CC^T[ACGT]AGG
MroI 521 TCCGGA T^CCGGA
NcoI 1577 CCATGG C^CATGG
PflMI 1593 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 443 CAGCTG CAG^CTG
SacI 1603 GAGCTC GAGCT^C
SfiI 943 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 966 CTTGAG C^T[CT][GA]AG
SphI 1565 GCATGC GCATG^C
Van91I 1593 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1290 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 565 GGCGTC G[GA]^CG[CT]C
1264 GACGTC
BalI 1190 TGGCCA TGG^CCA
1514 TGGCCA
BsaHI 565 GGCGTC G[GA]^CG[CT]C
1264 GACGTC
BsaWI 521 TCCGGA [AT]^CCGG[AT]
1279 ACCGGA
BsgI 1117 GTGCAG GTGCAG(16/14)
1504 GTGCAG
BsrDI 795 CATTGC GCAATG(2/0)
1350 CATTGC
BstXI 458 CCAAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1571 CCATGACCATGG
DpnII 750 GATC ^GATC
1473 GATC
EcoT14I 464 CCTTGG C^C[AT][AT]GG
1577 CCATGG
HgaI 910 GACGC GACGC(5/10)
566 GCGTC
Hin1I 565 GGCGTC G[GA]^CG[CT]C
1264 GACGTC
KspI 37 CCGCGG CCGC^GG
1146 CCGCGG
MaeII 1265 ACGT A^CGT
1271 ACGT
MboI 750 GATC ^GATC
1473 GATC
MluNI 1190 TGGCCA TGG^CCA
1514 TGGCCA
MscI 1190 TGGCCA TGG^CCA
1514 TGGCCA
NdeII 750 GATC ^GATC
1473 GATC
NspI 1496 GCATGT [GA]CATG^[CT]
1565 GCATGC
SacII 37 CCGCGG CCGC^GG
1146 CCGCGG
SapI 1293 GCTCTTC GCTCTTC(1/4)
1449 GCTCTTC
Sau3AI 750 GATC ^GATC
1473 GATC
SexAI 1052 ACCAGGT A^CC[AT]GGT
1592 ACCAGGT
StyI 464 CCTTGG C^C[AT][AT]GG
1577 CCATGG
Tsp45I 353 GTGAC ^GT[GC]AC
1400 GTGAC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PpuMI PstI RsaI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I