Restriction map for
pF1KE3369
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BlnI BsaAI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoNI
FseI FspI HgaI Hin1I HindIII
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2773 GTCTAC GT^[AC][GT]AC
Alw44I 1302 GTGCAC G^TGCAC
AlwNI 3028 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3085 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3037 AGCGCT AGC^GCT
ApaI 553 GGGCCC GGGCC^C
ApaLI 1302 GTGCAC G^TGCAC
BlpI 51 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
BsaBI 1608 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2738 ACCGGA [AT]^CCGG[AT]
BsmBI 3151 GAGACG CGTCTC(1/5)
BsmI 2645 GCATTC GAATGC(1/-1)
BspHI 1862 TCATGA T^CATGA
BsrFI 1847 GCCGGT [GA]^CCGG[CT]
BstBI 2538 TTCGAA TT^CGAA
BstEII 696 GGTCACC G^GT[ACGT]ACC
BstPI 696 GGTCACC G^GT[ACGT]ACC
Bsu36I 3085 CCTGAGG CC^T[ACGT]AGG
CelII 51 GCTGAGC GC^T[ACGT]AGC
CfoI 3038 GCGC GCG^C
Cfr10I 1847 GCCGGT [GA]^CCGG[CT]
DraI 621 TTTAAA TTT^AAA
DraIII 2621 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2323 GACTCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3037 AGCGCT AGC^GCT
Eco81I 3085 CCTGAGG CC^T[ACGT]AGG
EcoO65I 696 GGTCACC G^GT[ACGT]ACC
EcoRI 123 GAATTC G^AATTC
HaeII 3037 AGCGCT [GA]GCGC^[CT]
HhaI 3038 GCGC GCG^C
HinP1I 3038 GCGC G^CGC
HpaI 1227 GTTAAC GTT^AAC
MaeII 2468 ACGT A^CGT
MamI 1608 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1272 CCATGG C^CATGG
NspI 2936 GCATGC [GA]CATG^[CT]
NspV 2538 TTCGAA TT^CGAA
PflMI 1765 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1586 CTGCAG CTGCA^G
RcaI 1862 TCATGA T^CATGA
SacI 195 GAGCTC GAGCT^C
SapI 1206 GAAGAGC GCTCTTC(1/4)
SexAI 3245 ACCTGGT A^CC[AT]GGT
SfuI 2538 TTCGAA TT^CGAA
SpeI 1776 ACTAGT A^CTAGT
SphI 2936 GCATGC GCATG^C
SspI 2258 AATATT AAT^ATT
StuI 2364 AGGCCT AGG^CCT
Van91I 1765 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 645 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 501 GGTACC G^GTACC
2735 GGTACC
Asp718I 501 GGTACC G^GTACC
2735 GGTACC
AspHI 195 GAGCTC G[AT]GC[AT]^C
1302 GTGCAC
AspI 1255 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3076 GACCCTGTC
BanII 195 GAGCTC G[GA]GC[CT]^C
553 GGGCCC
BcgI 864 CGAATGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1730 CGATGCAGCTGC
BclI 1266 TGATCA T^GATCA
1613 TGATCA
BcnI 1698 CCCGG CC^[GC]GG
2689 CCCGG
BglI 268 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
874 GCCCGCCAGGC
BglII 2498 AGATCT A^GATCT
2660 AGATCT
BsgI 1989 CTGCAC GTGCAG(16/14)
115 GTGCAG
BsiHKAI 195 GAGCTC G[AT]GC[AT]^C
1302 GTGCAC
Bsp1407I 290 TGTACA T^GTACA
815 TGTACA
BspMI 504 ACCTGC ACCTGC(4/8)
983 ACCTGC
BsrGI 290 TGTACA T^GTACA
815 TGTACA
BstXI 803 CCATCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCACTGATGTGG
EcoRV 1038 GATATC GAT^ATC
2116 GATATC
EcoT22I 2530 ATGCAT ATGCA^T
2957 ATGCAT
FauI 875 CCCGC CCCGC(4/6)
1017 GCGGG
FbaI 1266 TGATCA T^GATCA
1613 TGATCA
KpnI 501 GGTACC GGTAC^C
2735 GGTACC
NciI 1698 CCCGG CC^[GC]GG
2689 CCCGG
NsiI 2530 ATGCAT ATGCA^T
2957 ATGCAT
PflFI 1255 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3076 GACCCTGTC
PpuMI 538 GGGACCT [GA]G^G[AT]CC[CT]
3095 AGGACCT
SfcI 548 CTGTAG C^T[GA][CT]AG
1586 CTGCAG
SmlI 1232 CTCAAG C^T[CT][GA]AG
1749 CTCAAG
SspBI 290 TGTACA T^GTACA
815 TGTACA
Tth111I 1255 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3076 GACCCTGTC
XcmI 1247 CCATTCTGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1272 CCATGGCTCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BalI
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI