Restriction map for
pF1KB9900
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I AspEI
BalI BbsI BbvCI BcgI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tsp509I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 698 ACGTGT A^C[GA][CT]GT
Alw44I 361 GTGCAC G^TGCAC
Aor51HI 428 AGCGCT AGC^GCT
ApaLI 361 GTGCAC G^TGCAC
AspHI 361 GTGCAC G[AT]GC[AT]^C
AvaI 110 CCCGAG C^[CT]CG[GA]G
AviII 1097 TGCGCA TGC^GCA
AvrII 133 CCTAGG C^CTAGG
BbeI 38 GGCGCC GGCGC^C
BbrPI 913 CACGTG CAC^GTG
BciVI 490 GGATAC GTATCC(6/5)
BfaI 134 CTAG C^TAG
BlnI 133 CCTAGG C^CTAGG
BsaAI 913 CACGTG [CT]AC^GT[GA]
BsiEI 302 CGGCCG CG[GA][CT]^CG
BsiHKAI 361 GTGCAC G[AT]GC[AT]^C
BsoBI 110 CCCGAG C^[CT]CG[GA]G
Bsp1407I 380 TGTACA T^GTACA
BsrBI 586 CCGCTC CCGCTC(-3/-3)
BsrFI 467 GCCGGC [GA]^CCGG[CT]
BsrGI 380 TGTACA T^GTACA
BstAPI 1052 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 935 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 467 GCCGGC [GA]^CCGG[CT]
EagI 302 CGGCCG C^GGCCG
EclXI 302 CGGCCG C^GGCCG
Eco47III 428 AGCGCT AGC^GCT
Eco52I 302 CGGCCG C^GGCCG
EcoRV 373 GATATC GAT^ATC
FspI 1097 TGCGCA TGC^GCA
KasI 38 GGCGCC G^GCGCC
MaeI 134 CTAG C^TAG
MaeIII 163 GTCAC ^GT[ACGT]AC
MseI 254 TTAA T^TAA
NaeI 467 GCCGGC GCC^GGC
NarI 38 GGCGCC GG^CGCC
NgoMIV 467 GCCGGC G^CCGGC
PmaCI 913 CACGTG CAC^GTG
PmlI 913 CACGTG CAC^GTG
SfaNI 118 GATGC GCATC(5/9)
SfoI 38 GGCGCC GGC^GCC
SmlI 688 CTCAAG C^T[CT][GA]AG
SspBI 380 TGTACA T^GTACA
TaqI 779 TCGA T^CGA
TfiI 1078 GAATC G^A[AT]TC
Tru9I 254 TTAA T^TAA
Tsp45I 163 GTCAC ^GT[GC]AC
TthHB8I 779 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 950 GGTACC G^GTACC
1085 GGTACC
AccII 300 CGCG CG^CG
351 CGCG
AcyI 38 GGCGCC G[GA]^CG[CT]C
901 GACGCC
ApaI 12 GGGCCC GGGCC^C
861 GGGCCC
Asp718I 950 GGTACC G^GTACC
1085 GGTACC
AspI 349 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
747 GACGCTGTC
BamHI 3 GGATCC G^GATCC
127 GGATCC
BsaHI 38 GGCGCC G[GA]^CG[CT]C
901 GACGCC
BspMI 98 ACCTGC ACCTGC(4/8)
953 ACCTGC
BstF5I 909 CATCC GGATG(2/0)
918 GGATG
BstUI 300 CGCG CG^CG
351 CGCG
DdeI 282 CTCAG C^T[ACGT]AG
988 CTGAG
EcoNI 133 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
723 CCTGCCCAAGG
FokI 909 CATCC GGATG(9/13)
918 GGATG
HaeII 38 GGCGCC [GA]GCGC^[CT]
428 AGCGCT
Hin1I 38 GGCGCC G[GA]^CG[CT]C
901 GACGCC
KpnI 950 GGTACC GGTAC^C
1085 GGTACC
MboII 89 TCTTC GAAGA(8/7)
533 TCTTC
MvnI 300 CGCG CG^CG
351 CGCG
PflFI 349 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
747 GACGCTGTC
PpuMI 181 GGGTCCC [GA]G^G[AT]CC[CT]
938 AGGACCT
PvuII 67 CAGCTG CAG^CTG
1032 CAGCTG
SbfI 675 CCTGCAGG CCTGCA^GG
954 CCTGCAGG
Sse8387I 675 CCTGCAGG CCTGCA^GG
954 CCTGCAGG
StuI 836 AGGCCT AGG^CCT
1055 AGGCCT
Tth111I 349 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
747 GACGCTGTC
XcmI 25 CCACAGTGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
602 CCAAGACCGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TseI TspRI XhoII