Restriction map for
pF1KB9019
##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
Acc65I AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AvrII BlnI
BsiWI Bsp1407I BsrGI BssHII Bst1107I
BstZ17I CpoI DrdI Eco47III FseI
HpaI KpnI MfeI MluI MunI
NdeI NheI NotI PacI PaeR7I
PinAI PmeI PvuI RsrII SalI
ScaI SgrAI SnaBI SpeI SspBI
SspI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3823 GACGTC GACGT^C
AclI 2803 AACGTT AA^CGTT
AflII 910 CTTAAG C^TTAAG
AhdI 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4754 TGCGCA TGC^GCA
BbeI 5494 GGCGCC GGCGC^C
BcgI 2535 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5211 GTATCC GTATCC(6/5)
BfaI 6784 CTAG C^TAG
BfrI 910 CTTAAG C^TTAAG
BsmBI 7093 GAGACG CGTCTC(1/5)
Eam1105I 363 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5956 ATGCAT ATGCA^T
FspI 4754 TGCGCA TGC^GCA
HaeII 5494 GGCGCC [GA]GCGC^[CT]
HincII 2620 GTTGAC GT[CT]^[GA]AC
HindII 2620 GTTGAC GT[CT]^[GA]AC
HindIII 1361 AAGCTT A^AGCTT
KasI 5494 GGCGCC G^GCGCC
KspI 3366 CCGCGG CCGC^GG
MaeI 6784 CTAG C^TAG
NarI 5494 GGCGCC GG^CGCC
NsiI 5956 ATGCAT ATGCA^T
PshAI 633 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2803 AACGTT AA^CGTT
SacII 3366 CCGCGG CCGC^GG
SbfI 7029 CCTGCAGG CCTGCA^GG
SfiI 1131 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5494 GGCGCC GGC^GCC
SmaI 5450 CCCGGG CCC^GGG
SphI 824 GCATGC GCATG^C
Sse8387I 7029 CCTGCAGG CCTGCA^GG
SwaI 5574 ATTTAAAT ATTT^AAAT
XmaCI 5450 CCCGGG C^CCGGG
XmaI 5450 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 1142 CCTTAGG CC^T[ACGT]AGG
3465 CCTCAGG
AseI 2448 ATTAAT AT^TAAT
3795 ATTAAT
AsnI 2448 ATTAAT AT^TAAT
3795 ATTAAT
AspI 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7225 GACTCTGTC
BamHI 3362 GGATCC G^GATCC
5398 GGATCC
BbrPI 5536 CACGTG CAC^GTG
7054 CACGTG
BglI 1132 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1615 GCCCCGCTGGC
BglII 5011 AGATCT A^GATCT
6726 AGATCT
BlpI 3633 GCTGAGC GC^T[ACGT]AGC
7318 GCTGAGC
Bpu1102I 3633 GCTGAGC GC^T[ACGT]AGC
7318 GCTGAGC
BsaI 631 GAGACC GGTCTC(1/5)
2224 GAGACC
BsmI 1533 GAATGC GAATGC(1/-1)
5462 GCATTC
BspDI 3562 ATCGAT AT^CGAT
6751 ATCGAT
BssSI 2077 CACGAG CACGAG(-5/-1)
551 CTCGTG
BstBI 2142 TTCGAA TT^CGAA
4672 TTCGAA
BstEII 894 GGTGACC G^GT[ACGT]ACC
5790 GGTGACC
BstPI 894 GGTGACC G^GT[ACGT]ACC
5790 GGTGACC
Bsu36I 1142 CCTTAGG CC^T[ACGT]AGG
3465 CCTCAGG
CelII 3633 GCTGAGC GC^T[ACGT]AGC
7318 GCTGAGC
ClaI 3562 ATCGAT AT^CGAT
6751 ATCGAT
DraI 5185 TTTAAA TTT^AAA
5575 TTTAAA
EagI 1802 CGGCCG C^GGCCG
6912 CGGCCG
EclXI 1802 CGGCCG C^GGCCG
6912 CGGCCG
Eco52I 1802 CGGCCG C^GGCCG
6912 CGGCCG
Eco81I 1142 CCTTAGG CC^T[ACGT]AGG
3465 CCTCAGG
EcoNI 404 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6999 CCTCCTGGAGG
EcoO65I 894 GGTGACC G^GT[ACGT]ACC
5790 GGTGACC
NruI 1554 TCGCGA TCG^CGA
6843 TCGCGA
NspV 2142 TTCGAA TT^CGAA
4672 TTCGAA
PflFI 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7225 GACTCTGTC
PmaCI 5536 CACGTG CAC^GTG
7054 CACGTG
PmlI 5536 CACGTG CAC^GTG
7054 CACGTG
PshBI 2448 ATTAAT AT^TAAT
3795 ATTAAT
SapI 4249 GAAGAGC GCTCTTC(1/4)
4056 GCTCTTC
SfuI 2142 TTCGAA TT^CGAA
4672 TTCGAA
Tth111I 7009 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7225 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoRV EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII