Restriction map for
pF1KE9645
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII Acc65I AccIII AclI AcyI
Aor51HI ApaI AscI Asp718I AviII
AvrII BalI BanI BbeI BcgI
BcnI BglI BlnI BsaHI BseAI
BsiWI BspDI BspEI BsrBI BssHII
BssSI Bst1107I BstZ17I CfoI ClaI
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI NaeI NarI NciI
NgoMIV NotI NruI PacI PaeR7I
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 7124 GTAGAC GT^[AC][GT]AC
AccII 478 CGCG CG^CG
AgeI 1465 ACCGGT A^CCGGT
AhdI 524 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 7421 GTGCAC G^TGCAC
ApaLI 7421 GTGCAC G^TGCAC
AspEI 524 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5885 CTCGGG C^[CT]CG[GA]G
BamHI 5311 GGATCC G^GATCC
BbrPI 1970 CACGTG CAC^GTG
BglII 5881 AGATCT A^GATCT
BlpI 7 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7 GCTGAGC GC^T[ACGT]AGC
BsaAI 1970 CACGTG [CT]AC^GT[GA]
BsaI 1081 GAGACC GGTCTC(1/5)
BsaWI 1465 ACCGGT [AT]^CCGG[AT]
BsiEI 1996 CGACCG CG[GA][CT]^CG
BsmBI 4506 GAGACG CGTCTC(1/5)
BsoBI 5885 CTCGGG C^[CT]CG[GA]G
Bsp1407I 381 TGTACA T^GTACA
BspLU11I 2857 ACATGT A^CATGT
BsrFI 1465 ACCGGT [GA]^CCGG[CT]
BsrGI 381 TGTACA T^GTACA
BstEII 7300 GGTCACC G^GT[ACGT]ACC
BstPI 7300 GGTCACC G^GT[ACGT]ACC
BstUI 478 CGCG CG^CG
BstXI 761 CCACAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 7 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1465 ACCGGT [GA]^CCGG[CT]
DraIII 4584 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 524 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 7300 GGTCACC G^GT[ACGT]ACC
FauI 2311 GCGGG CCCGC(4/6)
HapII 1466 CCGG C^CGG
HpaII 1466 CCGG C^CGG
MfeI 251 CAATTG C^AATTG
MspI 1466 CCGG C^CGG
MunI 251 CAATTG C^AATTG
MvnI 478 CGCG CG^CG
NdeI 3257 CATATG CA^TATG
NheI 2202 GCTAGC G^CTAGC
PflFI 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1465 ACCGGT A^CCGGT
PmaCI 1970 CACGTG CAC^GTG
PmlI 1970 CACGTG CAC^GTG
PstI 5005 CTGCAG CTGCA^G
SapI 197 GCTCTTC GCTCTTC(1/4)
ScaI 5543 AGTACT AGT^ACT
SpeI 958 ACTAGT A^CTAGT
SspBI 381 TGTACA T^GTACA
Tth111I 823 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 1174 CTTAAG C^TTAAG
4489 CTTAAG
AflIII 1971 ACGTGT A^C[GA][CT]GT
2857 ACATGT
AocI 3085 CCTAAGG CC^T[ACGT]AGG
4984 CCTCAGG
BbvCI 3472 GCTGAGG CCTCAGC(-5/-2)
6031 GCTGAGG
BfrI 1174 CTTAAG C^TTAAG
4489 CTTAAG
BsaBI 3628 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7197 GATGTTAATC
BstAPI 354 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
719 GCAAGAAATGC
Bsu36I 3085 CCTAAGG CC^T[ACGT]AGG
4984 CCTCAGG
DsaI 5378 CCATGG C^C[GA][CT]GG
6509 CCATGG
Eco81I 3085 CCTAAGG CC^T[ACGT]AGG
4984 CCTCAGG
MamI 3628 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7197 GATGTTAATC
NcoI 5378 CCATGG C^CATGG
6509 CCATGG
PflMI 4779 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7384 CCAGTGGCTGG
PpuMI 6974 AGGACCT [GA]G^G[AT]CC[CT]
7452 AGGACCT
SacI 2397 GAGCTC GAGCT^C
6715 GAGCTC
Van91I 4779 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7384 CCAGTGGCTGG
XcmI 4686 CCATCAAGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5989 CCAGCTCCAGACTGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BciVI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstBI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HgaI HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI NspV PleI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI