Restriction map for
pF1KB5818
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsgI BsiWI
BspDI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SapI SbfI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tru9I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 370 GTCGAC GT^[AC][GT]AC
AccII 114 CGCG CG^CG
AccIII 126 TCCGGA T^CCGGA
AflIII 1106 ACATGT A^C[GA][CT]GT
AlwNI 272 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1212 CCTGAGG CC^T[ACGT]AGG
Asp700I 135 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 1409 CTAG C^TAG
BlpI 1308 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1308 GCTGAGC GC^T[ACGT]AGC
BsaI 1231 GAGACC GGTCTC(1/5)
BsaWI 126 TCCGGA [AT]^CCGG[AT]
BseAI 126 TCCGGA T^CCGGA
BsiEI 549 CGGCCG CG[GA][CT]^CG
BsmBI 107 GAGACG CGTCTC(1/5)
BsmI 360 GAATGC GAATGC(1/-1)
Bsp1407I 1109 TGTACA T^GTACA
BspEI 126 TCCGGA T^CCGGA
BspLU11I 1106 ACATGT A^CATGT
BsrDI 324 CATTGC GCAATG(2/0)
BsrGI 1109 TGTACA T^GTACA
BstAPI 1065 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1255 GGTGACC G^GT[ACGT]ACC
BstPI 1255 GGTGACC G^GT[ACGT]ACC
BstUI 114 CGCG CG^CG
BstYI 906 GGATCT [GA]^GATC[CT]
Bsu36I 1212 CCTGAGG CC^T[ACGT]AGG
CelII 1308 GCTGAGC GC^T[ACGT]AGC
CfoI 113 GCGC GCG^C
EagI 549 CGGCCG C^GGCCG
EarI 349 GAAGAG CTCTTC(1/4)
EclXI 549 CGGCCG C^GGCCG
Eco52I 549 CGGCCG C^GGCCG
Eco81I 1212 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1255 GGTGACC G^GT[ACGT]ACC
HhaI 113 GCGC GCG^C
HinP1I 113 GCGC G^CGC
Ksp632I 349 GAAGAG CTCTTC(1/4)
MaeI 1409 CTAG C^TAG
MaeII 76 ACGT A^CGT
MflI 906 GGATCT [GA]^GATC[CT]
MroI 126 TCCGGA T^CCGGA
MvnI 114 CGCG CG^CG
NcoI 812 CCATGG C^CATGG
PpuMI 1079 GGGACCT [GA]G^G[AT]CC[CT]
SacI 319 GAGCTC GAGCT^C
SalI 370 GTCGAC G^TCGAC
ScaI 857 AGTACT AGT^ACT
SfiI 254 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 443 CCCGGG CCC^GGG
SmlI 713 CTCAAG C^T[CT][GA]AG
SphI 665 GCATGC GCATG^C
SspBI 1109 TGTACA T^GTACA
SspI 1124 AATATT AAT^ATT
TaqI 371 TCGA T^CGA
TthHB8I 371 TCGA T^CGA
XhoII 906 GGATCT [GA]^GATC[CT]
XmaCI 443 CCCGGG C^CCGGG
XmaI 443 CCCGGG C^CCGGG
XmnI 135 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 1278 GGGCCC GGGCC^C
1404 GGGCCC
AspHI 319 GAGCTC G[AT]GC[AT]^C
1322 GAGCAC
AvaI 102 CTCGGG C^[CT]CG[GA]G
443 CCCGGG
AvaII 483 GGTCC G^G[AT]CC
1080 GGACC
BalI 228 TGGCCA TGG^CCA
506 TGGCCA
BanI 616 GGTGCC G^G[CT][GA]CC
723 GGTGCC
BbsI 942 GAAGAC GAAGAC(2/6)
1219 GAAGAC
BglI 255 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
711 GCCTCAAGGGC
BpmI 728 CTGGAG CTGGAG(16/14)
1228 CTGGAG
BpuAI 942 GAAGAC GAAGAC(2/6)
1219 GAAGAC
BsiHKAI 319 GAGCTC G[AT]GC[AT]^C
1322 GAGCAC
BsoBI 102 CTCGGG C^[CT]CG[GA]G
443 CCCGGG
BspMI 863 ACCTGC ACCTGC(4/8)
1082 ACCTGC
EcoNI 624 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
757 CCTCACCGAGG
HincII 370 GTCGAC GT[CT]^[GA]AC
583 GTCAAC
HindII 370 GTCGAC GT[CT]^[GA]AC
583 GTCAAC
MluNI 228 TGGCCA TGG^CCA
506 TGGCCA
MscI 228 TGGCCA TGG^CCA
506 TGGCCA
MslI 1134 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1378 CACTACGATG
MspA1I 28 CCGCTG C[AC]G^C[GT]G
417 CAGCGG
PflMI 1282 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGTACATGG
PstI 169 CTGCAG CTGCA^G
1028 CTGCAG
SfcI 169 CTGCAG C^T[GA][CT]AG
1028 CTGCAG
TfiI 331 GAATC G^A[AT]TC
954 GAATC
Van91I 1282 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGTACATGG
XcmI 335 CCAAGAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
812 CCATGGTTCGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StuI StyI TseI Tsp45I
Tsp509I TspRI