Restriction map for
pF1KE2348
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AcyI Alw44I Aor51HI ApaLI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BbvCI BcgI BglII
BlnI BsaHI BsiWI BsmBI Bsp1407I
BspLU11I BsrGI BssHII CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRI
FseI FspI Hin1I HindIII KasI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspI PacI PflMI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
Van91I
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 3602 GGTACC G^GTACC
AccII 1153 CGCG CG^CG
AclI 2986 AACGTT AA^CGTT
AflII 787 CTTAAG C^TTAAG
AgeI 736 ACCGGT A^CCGGT
AhdI 394 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1986 CCTGAGG CC^T[ACGT]AGG
Asp700I 1420 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3602 GGTACC G^GTACC
AspEI 394 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3504 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2000 TGGCCA TGG^CCA
BclI 560 TGATCA T^GATCA
BfrI 787 CTTAAG C^TTAAG
BlpI 3937 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3937 GCTCAGC GC^T[ACGT]AGC
BsaAI 3273 TACGTG [CT]AC^GT[GA]
BsaBI 2973 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 124 CGACCG CG[GA][CT]^CG
BspDI 1741 ATCGAT AT^CGAT
BspHI 2264 TCATGA T^CATGA
BssSI 415 CACGAG CACGAG(-5/-1)
Bst1107I 618 GTATAC GTA^TAC
BstAPI 2484 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2867 TTCGAA TT^CGAA
BstUI 1153 CGCG CG^CG
BstZ17I 618 GTATAC GTA^TAC
Bsu36I 1986 CCTGAGG CC^T[ACGT]AGG
CelII 3937 GCTCAGC GC^T[ACGT]AGC
ClaI 1741 ATCGAT AT^CGAT
DraI 2316 TTTAAA TTT^AAA
Eam1105I 394 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1986 CCTGAGG CC^T[ACGT]AGG
EcoRV 7 GATATC GAT^ATC
EcoT22I 193 ATGCAT ATGCA^T
FbaI 560 TGATCA T^GATCA
HaeII 2066 AGCGCC [GA]GCGC^[CT]
HpaI 3414 GTTAAC GTT^AAC
KpnI 3602 GGTACC GGTAC^C
MamI 2973 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2000 TGGCCA TGG^CCA
MscI 2000 TGGCCA TGG^CCA
MvnI 1153 CGCG CG^CG
NsiI 193 ATGCAT ATGCA^T
NspV 2867 TTCGAA TT^CGAA
PaeR7I 2681 CTCGAG C^TCGAG
PflFI 3504 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 736 ACCGGT A^CCGGT
PshAI 2425 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2986 AACGTT AA^CGTT
RcaI 2264 TCATGA T^CATGA
SapI 463 GAAGAGC GCTCTTC(1/4)
ScaI 2735 AGTACT AGT^ACT
SfuI 2867 TTCGAA TT^CGAA
SmaI 2087 CCCGGG CCC^GGG
SwaI 2315 ATTTAAAT ATTT^AAAT
Tth111I 3504 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1842 TCTAGA T^CTAGA
XhoI 2681 CTCGAG C^TCGAG
XmaCI 2087 CCCGGG C^CCGGG
XmaI 2087 CCCGGG C^CCGGG
XmnI 1420 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 618 GTATAC GT^[AC][GT]AC
2542 GTCTAC
AccIII 272 TCCGGA T^CCGGA
2183 TCCGGA
ApaI 2562 GGGCCC GGGCC^C
3055 GGGCCC
BamHI 1898 GGATCC G^GATCC
2861 GGATCC
BanI 2293 GGCACC G^G[CT][GA]CC
3602 GGTACC
BciVI 1514 GTATCC GTATCC(6/5)
3637 GTATCC
BseAI 272 TCCGGA T^CCGGA
2183 TCCGGA
BspEI 272 TCCGGA T^CCGGA
2183 TCCGGA
BsrBI 1663 GAGCGG CCGCTC(-3/-3)
2055 GAGCGG
BsrFI 736 ACCGGT [GA]^CCGG[CT]
3828 GCCGGT
BstEII 1245 GGTGACC G^GT[ACGT]ACC
3576 GGTGACC
BstPI 1245 GGTGACC G^GT[ACGT]ACC
3576 GGTGACC
Cfr10I 736 ACCGGT [GA]^CCGG[CT]
3828 GCCGGT
DraIII 295 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2628 CACCTGGTG
EaeI 1043 TGGCCG [CT]^GGCC[GA]
2000 TGGCCA
EcoNI 213 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2434 CCTAAAACAGG
EcoO65I 1245 GGTGACC G^GT[ACGT]ACC
3576 GGTGACC
MroI 272 TCCGGA T^CCGGA
2183 TCCGGA
NdeI 1261 CATATG CA^TATG
2728 CATATG
SacI 379 GAGCTC GAGCT^C
823 GAGCTC
SexAI 2237 ACCTGGT A^CC[AT]GGT
2629 ACCTGGT
SfiI 354 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1884 GGCCCAGCAGGCC
StuI 1892 AGGCCT AGG^CCT
1990 AGGCCT
XcmI 758 CCATCCTAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4067 CCACAGCTGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII