Restriction map for
pF1KB5948
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 1629 CCGC CCGC(-3/-1)
Alw44I 437 GTGCAC G^TGCAC
AocI 1291 CCTGAGG CC^T[ACGT]AGG
ApaLI 437 GTGCAC G^TGCAC
AvaI 1183 CTCGGG C^[CT]CG[GA]G
BalI 881 TGGCCA TGG^CCA
BanI 1287 GGCACC G^G[CT][GA]CC
BanII 1441 GAGCTC G[GA]GC[CT]^C
BciVI 445 GGATAC GTATCC(6/5)
BsgI 1132 CTGCAC GTGCAG(16/14)
BsmFI 785 GGGAC GGGAC(10/14)
BsoBI 1183 CTCGGG C^[CT]CG[GA]G
Bsp1407I 698 TGTACA T^GTACA
BspHI 947 TCATGA T^CATGA
BsrGI 698 TGTACA T^GTACA
BstAPI 1103 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 283 CCAGAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1291 CCTGAGG CC^T[ACGT]AGG
CfoI 198 GCGC GCG^C
DraII 785 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 107 CCATGG C^C[GA][CT]GG
EaeI 881 TGGCCA [CT]^GGCC[GA]
Eco81I 1291 CCTGAGG CC^T[ACGT]AGG
EcoO109I 785 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1330 GATATC GAT^ATC
HaeII 197 AGCGCC [GA]GCGC^[CT]
HhaI 198 GCGC GCG^C
HinP1I 198 GCGC G^CGC
HincII 394 GTCAAC GT[CT]^[GA]AC
HindII 394 GTCAAC GT[CT]^[GA]AC
MfeI 1355 CAATTG C^AATTG
MluNI 881 TGGCCA TGG^CCA
MscI 881 TGGCCA TGG^CCA
MslI 1260 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1355 CAATTG C^AATTG
NcoI 107 CCATGG C^CATGG
PpuMI 785 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1101 CTGCAG CTGCA^G
RcaI 947 TCATGA T^CATGA
SacI 1441 GAGCTC GAGCT^C
ScaI 691 AGTACT AGT^ACT
SexAI 1142 ACCAGGT A^CC[AT]GGT
SfcI 1101 CTGCAG C^T[GA][CT]AG
SmlI 1498 CTTGAG C^T[CT][GA]AG
SspBI 698 TGTACA T^GTACA
XbaI 357 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 1149 GATCC GGATC(4/5)
1522 GGATC
AlwNI 479 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
836 CAGGTACTG
AseI 924 ATTAAT AT^TAAT
931 ATTAAT
AsnI 924 ATTAAT AT^TAAT
931 ATTAAT
AspHI 437 GTGCAC G[AT]GC[AT]^C
1441 GAGCTC
AvaII 786 GGACC G^G[AT]CC
1318 GGTCC
BfaI 358 CTAG C^TAG
856 CTAG
BglII 206 AGATCT A^GATCT
749 AGATCT
BsaI 1066 GAGACC GGTCTC(1/5)
1337 GGTCTC
BsiHKAI 437 GTGCAC G[AT]GC[AT]^C
1441 GAGCTC
BsiYI 157 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1274 CCCCATATTGG
BslI 157 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1274 CCCCATATTGG
Cac8I 1112 GCCTGC GC[ACGT]^[ACGT]GC
1116 GCAAGC
DraI 1485 TTTAAA TTT^AAA
1554 TTTAAA
EcoRI 268 GAATTC G^AATTC
278 GAATTC
EcoT14I 107 CCATGG C^C[AT][AT]GG
1529 CCAAGG
HindIII 1118 AAGCTT A^AGCTT
1310 AAGCTT
MaeI 358 CTAG C^TAG
856 CTAG
MspA1I 1094 CAGCTG C[AC]G^C[GT]G
1466 CAGCTG
PleI 1302 GACTC GAGTC(4/5)
1584 GACTC
PshBI 924 ATTAAT AT^TAAT
931 ATTAAT
PvuII 1094 CAGCTG CAG^CTG
1466 CAGCTG
SspI 970 AATATT AAT^ATT
1174 AATATT
StyI 107 CCATGG C^C[AT][AT]GG
1529 CCAAGG
TaqI 466 TCGA T^CGA
1305 TCGA
TspRI 44 CACTG CA[GC]TG[ACGT][ACGT]^
273 CACTG
TthHB8I 466 TCGA T^CGA
1305 TCGA
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
XhoII