Restriction map for
pF1KE0700
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
AviII AvrII BamHI BclI BfrI
BlnI BsaBI BsiWI BsmI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoRI
EcoRV FbaI FseI FspI MamI
MfeI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SalI ScaI SexAI SfuI
SnaBI SpeI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 3409 GTCTAC GT^[AC][GT]AC
Alw44I 4932 GTGCAC G^TGCAC
ApaLI 4932 GTGCAC G^TGCAC
AspI 3177 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3991 CACGTG CAC^GTG
BglII 3419 AGATCT A^GATCT
Bsp1407I 4817 TGTACA T^GTACA
BsrDI 1067 GCAATG GCAATG(2/0)
BsrGI 4817 TGTACA T^GTACA
BssSI 1261 CACGAG CACGAG(-5/-1)
DraIII 2602 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3455 CGGCCG C^GGCCG
EclXI 3455 CGGCCG C^GGCCG
Eco52I 3455 CGGCCG C^GGCCG
HindIII 144 AAGCTT A^AGCTT
HpaI 112 GTTAAC GTT^AAC
KspI 4944 CCGCGG CCGC^GG
MluI 200 ACGCGT A^CGCGT
MseI 113 TTAA T^TAA
NcoI 4266 CCATGG C^CATGG
PflFI 3177 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1926 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3991 CACGTG CAC^GTG
PmlI 3991 CACGTG CAC^GTG
PvuI 4721 CGATCG CGAT^CG
PvuII 1838 CAGCTG CAG^CTG
SacII 4944 CCGCGG CCGC^GG
SapI 2815 GAAGAGC GCTCTTC(1/4)
SfiI 2655 GGCCGTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4024 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 4817 TGTACA T^GTACA
TfiI 922 GATTC G^A[AT]TC
Tru9I 113 TTAA T^TAA
Tth111I 3177 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1926 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 2147 GGTACC G^GTACC
4739 GGTACC
Asp700I 2637 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2815 GAAGAGCTTC
Asp718I 2147 GGTACC G^GTACC
4739 GGTACC
BbeI 4028 GGCGCC GGCGC^C
4799 GGCGCC
BcgI 2448 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2552 GCAGGGCCTTCG
BciVI 262 GGATAC GTATCC(6/5)
3676 GGATAC
BglI 1399 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2656 GCCGTCCAGGC
BsaAI 529 TACGTG [CT]AC^GT[GA]
3991 CACGTG
BspLU11I 410 ACATGT A^CATGT
4814 ACATGT
EcoT22I 3486 ATGCAT ATGCA^T
4981 ATGCAT
KasI 4028 GGCGCC G^GCGCC
4799 GGCGCC
KpnI 2147 GGTACC GGTAC^C
4739 GGTACC
NarI 4028 GGCGCC GG^CGCC
4799 GGCGCC
NsiI 3486 ATGCAT ATGCA^T
4981 ATGCAT
SacI 1336 GAGCTC GAGCT^C
3976 GAGCTC
SbfI 3018 CCTGCAGG CCTGCA^GG
4152 CCTGCAGG
SfoI 4028 GGCGCC GGC^GCC
4799 GGCGCC
SphI 249 GCATGC GCATG^C
3479 GCATGC
Sse8387I 3018 CCTGCAGG CCTGCA^GG
4152 CCTGCAGG
StuI 2913 AGGCCT AGG^CCT
3092 AGGCCT
XmnI 2637 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2815 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI