Restriction map for
pF1KB5185
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 17 GGTACC G^GTACC
AccI 634 GTAGAC GT^[AC][GT]AC
AccII 640 CGCG CG^CG
AocI 1179 CCTCAGG CC^T[ACGT]AGG
Asp718I 17 GGTACC G^GTACC
AspHI 664 GTGCTC G[AT]GC[AT]^C
AvaI 780 CCCGGG C^[CT]CG[GA]G
BalI 1157 TGGCCA TGG^CCA
BamHI 284 GGATCC G^GATCC
BsgI 730 CTGCAC GTGCAG(16/14)
BsiHKAI 664 GTGCTC G[AT]GC[AT]^C
BsoBI 780 CCCGGG C^[CT]CG[GA]G
BssSI 362 CACGAG CACGAG(-5/-1)
BstAPI 763 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 204 GGTCACC G^GT[ACGT]ACC
BstPI 204 GGTCACC G^GT[ACGT]ACC
BstUI 640 CGCG CG^CG
Bsu36I 1179 CCTCAGG CC^T[ACGT]AGG
DraIII 1021 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1010 GAAGAG CTCTTC(1/4)
Eco81I 1179 CCTCAGG CC^T[ACGT]AGG
EcoO65I 204 GGTCACC G^GT[ACGT]ACC
EcoT14I 524 CCAAGG C^C[AT][AT]GG
HinfI 334 GACTC G^A[ACGT]TC
KpnI 17 GGTACC GGTAC^C
Ksp632I 1010 GAAGAG CTCTTC(1/4)
MaeII 374 ACGT A^CGT
MluNI 1157 TGGCCA TGG^CCA
MscI 1157 TGGCCA TGG^CCA
MslI 495 CACACGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 640 CGCG CG^CG
PleI 334 GACTC GAGTC(4/5)
SmaI 780 CCCGGG CCC^GGG
StyI 524 CCAAGG C^C[AT][AT]GG
TaqI 61 TCGA T^CGA
TspRI 653 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 61 TCGA T^CGA
XmaCI 780 CCCGGG C^CCGGG
XmaI 780 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 18 GTAC GT^AC
136 GTAC
ApaI 694 GGGCCC GGGCC^C
1079 GGGCCC
BsrBI 106 GAGCGG CCGCTC(-3/-3)
256 GAGCGG
BstF5I 1038 CATCC GGATG(2/0)
260 GGATG
BstXI 297 CCAGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1160 CCATGAAGCTGG
CfoI 25 GCGC GCG^C
977 GCGC
DsaI 419 CCGTGG C^C[GA][CT]GG
797 CCGTGG
EaeI 1061 TGGCCG [CT]^GGCC[GA]
1157 TGGCCA
EcoNI 338 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
903 CCTGTCTGAGG
FokI 1038 CATCC GGATG(9/13)
260 GGATG
HaeII 24 GGCGCT [GA]GCGC^[CT]
976 AGCGCC
HhaI 25 GCGC GCG^C
977 GCGC
HinP1I 25 GCGC G^CGC
977 GCGC
MaeIII 205 GTCAC ^GT[ACGT]AC
610 GTGAC
MboII 237 GAAGA GAAGA(8/7)
1010 GAAGA
PflMI 614 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCAGCAGCTGG
PpuMI 300 GGGACCT [GA]G^G[AT]CC[CT]
449 GGGACCC
PvuII 689 CAGCTG CAG^CTG
721 CAGCTG
RsaI 18 GTAC GT^AC
136 GTAC
SfaNI 443 GCATC GCATC(5/9)
1037 GCATC
SfcI 892 CTACAG C^T[GA][CT]AG
998 CTACAG
Tsp45I 205 GTCAC ^GT[GC]AC
610 GTGAC
Van91I 614 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCAGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HpaII HphI ItaI
MboI MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SmlI
TseI XhoII