Restriction map for
pF1KE1092
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BfrI BglI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspHI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRV FseI FspI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 960 GGCGTC G[GA]^CG[CT]C
AocI 3737 CCTTAGG CC^T[ACGT]AGG
Aor51HI 4487 AGCGCT AGC^GCT
AspHI 4192 GAGCAC G[AT]GC[AT]^C
BciVI 99 GGATAC GTATCC(6/5)
BcnI 3602 CCGGG CC^[GC]GG
BlpI 2692 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2692 GCTCAGC GC^T[ACGT]AGC
BsaAI 4565 CACGTA [CT]AC^GT[GA]
BsaHI 960 GGCGTC G[GA]^CG[CT]C
BsiHKAI 4192 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1722 TGTACA T^GTACA
BsrBI 3798 CCGCTC CCGCTC(-3/-3)
BsrGI 1722 TGTACA T^GTACA
Bst1107I 1793 GTATAC GTA^TAC
BstAPI 1799 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1793 GTATAC GTA^TAC
Bsu36I 3737 CCTTAGG CC^T[ACGT]AGG
CelII 2692 GCTCAGC GC^T[ACGT]AGC
DsaI 2597 CCATGG C^C[GA][CT]GG
Eco47III 4487 AGCGCT AGC^GCT
Eco81I 3737 CCTTAGG CC^T[ACGT]AGG
EcoNI 1041 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2403 ATGCAT ATGCA^T
Hin1I 960 GGCGTC G[GA]^CG[CT]C
HpaI 4560 GTTAAC GTT^AAC
NciI 3602 CCGGG CC^[GC]GG
NcoI 2597 CCATGG C^CATGG
NdeI 1271 CATATG CA^TATG
NsiI 2403 ATGCAT ATGCA^T
SpeI 1234 ACTAGT A^CTAGT
SspBI 1722 TGTACA T^GTACA
StuI 3710 AGGCCT AGG^CCT
XbaI 3228 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1077 GTCTAC GT^[AC][GT]AC
1793 GTATAC
AhdI 148 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3820 GACATTCTGTC
AspEI 148 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3820 GACATTCTGTC
BalI 852 TGGCCA TGG^CCA
2438 TGGCCA
BanI 786 GGCACC G^G[CT][GA]CC
2943 GGTGCC
BanII 3556 GAGCCC G[GA]GC[CT]^C
3988 GAGCCC
BbvCI 1616 CCTCAGC CCTCAGC(-5/-2)
1751 CCTCAGC
BclI 426 TGATCA T^GATCA
1205 TGATCA
BseRI 1145 CTCCTC GAGGAG(10/8)
1937 GAGGAG
BsmBI 2002 CGTCTC CGTCTC(1/5)
4224 GAGACG
BspLU11I 3620 ACATGT A^CATGT
4064 ACATGT
Cac8I 1132 GCCCGC GC[ACGT]^[ACGT]GC
1408 GCCTGC
DraII 2619 AGGACCC [GA]G^G[ACGT]CC[CT]
3407 AGGACCT
EaeI 852 TGGCCA [CT]^GGCC[GA]
2438 TGGCCA
Eam1105I 148 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3820 GACATTCTGTC
EcoO109I 2619 AGGACCC [GA]G^G[ACGT]CC[CT]
3407 AGGACCT
FauI 1133 CCCGC CCCGC(4/6)
2565 CCCGC
FbaI 426 TGATCA T^GATCA
1205 TGATCA
HaeII 4114 GGCGCT [GA]GCGC^[CT]
4487 AGCGCT
HgaI 961 GCGTC GACGC(5/10)
2001 GCGTC
MluNI 852 TGGCCA TGG^CCA
2438 TGGCCA
MscI 852 TGGCCA TGG^CCA
2438 TGGCCA
PflMI 247 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCAGAATGTGG
PpuMI 2619 AGGACCC [GA]G^G[AT]CC[CT]
3407 AGGACCT
ScaI 2770 AGTACT AGT^ACT
3745 AGTACT
Van91I 247 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1377 CCAGAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI CfoI Cfr13I
DdeI DpnII DraI DraIII EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI