Restriction map for
pF1KB7687
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AvaI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HindIII HpaI HphI
KasI KpnI KspI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 709 CGCG CG^CG
AfaI 699 GTAC GT^AC
AhdI 394 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 26 GGATC GGATC(4/5)
ApaI 74 GGGCCC GGGCC^C
AspEI 394 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 553 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 713 TGCGCA TGC^GCA
BanI 904 GGCACC G^G[CT][GA]CC
BbvCI 21 GCTGAGG CCTCAGC(-5/-2)
BglI 592 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 40 CTGGAG CTGGAG(16/14)
BsaBI 111 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 701 ACCGGA [AT]^CCGG[AT]
BsmI 852 GAATGC GAATGC(1/-1)
BsrDI 1013 GCAATG GCAATG(2/0)
BstUI 709 CGCG CG^CG
BstXI 32 CCAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 714 GCGC GCG^C
DraIII 974 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 619 GACTATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 394 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 713 TGCGCA TGC^GCA
HhaI 714 GCGC GCG^C
HinP1I 714 GCGC G^CGC
HincII 628 GTCAAC GT[CT]^[GA]AC
HindII 628 GTCAAC GT[CT]^[GA]AC
MaeIII 635 GTGAC ^GT[ACGT]AC
MamI 111 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 155 TTAA T^TAA
MslI 687 CACGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 102 CAGCTG C[AC]G^C[GT]G
MvnI 709 CGCG CG^CG
PflFI 553 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 737 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 102 CAGCTG CAG^CTG
RsaI 699 GTAC GT^AC
SacI 676 GAGCTC GAGCT^C
SexAI 962 ACCAGGT A^CC[AT]GGT
SfcI 127 CTACAG C^T[GA][CT]AG
StuI 336 AGGCCT AGG^CCT
TaqI 656 TCGA T^CGA
Tru9I 155 TTAA T^TAA
Tsp45I 635 GTGAC ^GT[GC]AC
Tth111I 553 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 656 TCGA T^CGA
XcmI 518 CCATGTGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BbsI 364 GAAGAC GAAGAC(2/6)
402 GTCTTC
BcnI 561 CCGGG CC^[GC]GG
751 CCCGG
BfaI 235 CTAG C^TAG
485 CTAG
BpuAI 364 GAAGAC GAAGAC(2/6)
402 GTCTTC
BsmAI 383 GAGAC GTCTC(1/5)
524 GTCTC
BsmFI 551 GGGAC GGGAC(10/14)
563 GGGAC
BstAPI 167 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
929 GCAGCAAATGC
BstF5I 117 CATCC GGATG(2/0)
873 CATCC
EcoT14I 197 CCTTGG C^C[AT][AT]GG
349 CCAAGG
FokI 117 CATCC GGATG(9/13)
873 CATCC
MaeI 235 CTAG C^TAG
485 CTAG
MaeII 505 ACGT A^CGT
767 ACGT
NciI 561 CCGGG CC^[GC]GG
751 CCCGG
SfaNI 579 GATGC GCATC(5/9)
646 GCATC
StyI 197 CCTTGG C^C[AT][AT]GG
349 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI Ksp632I MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SmlI
TseI TspRI