Restriction map for
pF1KB8640
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
DpnII DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HinfI HpaI KpnI MamI MboI
MfeI MflI MluI MseI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI SacI SalI SapI
Sau3AI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 181 GACGTC GACGT^C
AccIII 213 TCCGGA T^CCGGA
AfaI 486 GTAC GT^AC
AscI 352 GGCGCGCC GG^CGCGCC
AviII 443 TGCGCA TGC^GCA
BalI 223 TGGCCA TGG^CCA
BanII 201 GAGCCC G[GA]GC[CT]^C
BbrPI 496 CACGTG CAC^GTG
BpmI 240 CTGGAG CTGGAG(16/14)
BsaI 337 GAGACC GGTCTC(1/5)
BsaWI 213 TCCGGA [AT]^CCGG[AT]
BseAI 213 TCCGGA T^CCGGA
BseRI 217 GAGGAG GAGGAG(10/8)
BsgI 551 CTGCAC GTGCAG(16/14)
BsmBI 505 CGTCTC CGTCTC(1/5)
BsmFI 392 GGGAC GGGAC(10/14)
BspEI 213 TCCGGA T^CCGGA
BssSI 373 CTCGTG CACGAG(-5/-1)
BstNI 341 CCAGG CC^[AT]GG
BstXI 368 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 414 CGGACCG CG^G[AT]CCG
DdeI 233 CTAAG C^T[ACGT]AG
DraII 314 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 508 CTCTTC CTCTTC(1/4)
EcoO109I 314 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 341 CCAGG ^CC[AT]GG
FspI 443 TGCGCA TGC^GCA
HphI 79 GGTGA GGTGA(8/7)
Ksp632I 508 CTCTTC CTCTTC(1/4)
MaeIII 80 GTGAC ^GT[ACGT]AC
MboII 509 TCTTC GAAGA(8/7)
MluNI 223 TGGCCA TGG^CCA
MroI 213 TCCGGA T^CCGGA
MscI 223 TGGCCA TGG^CCA
MslI 369 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 341 CCAGG CC^[AT]GG
NaeI 345 GCCGGC GCC^GGC
NgoMIV 345 GCCGGC G^CCGGC
NlaIII 606 CATG CATG^
PmaCI 496 CACGTG CAC^GTG
PmlI 496 CACGTG CAC^GTG
RsaI 486 GTAC GT^AC
RsrII 414 CGGACCG CG^G[AT]CCG
SbfI 570 CCTGCAGG CCTGCA^GG
Sse8387I 570 CCTGCAGG CCTGCA^GG
Tsp45I 80 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
274 GTCTAC
AluI 244 AGCT AG^CT
518 AGCT
AlwNI 400 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
447 CAGGGGCTG
AspHI 268 GTGCTC G[AT]GC[AT]^C
474 GTGCTC
AvaII 211 GGTCC G^G[AT]CC
415 GGACC
BanI 31 GGCGCC G^G[CT][GA]CC
148 GGCGCC
BbeI 31 GGCGCC GGCGC^C
148 GGCGCC
BfaI 28 CTAG C^TAG
439 CTAG
BsaAI 307 TACGTG [CT]AC^GT[GA]
496 CACGTG
BsiHKAI 268 GTGCTC G[AT]GC[AT]^C
474 GTGCTC
BsmAI 337 GAGAC GTCTC(1/5)
506 GTCTC
BsrFI 98 ACCGGC [GA]^CCGG[CT]
345 GCCGGC
BstAPI 399 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
495 GCACGTGCTGC
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
345 GCCGGC
DrdI 145 GACGGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
175 GACGGCGACGTC
KasI 31 GGCGCC G^GCGCC
148 GGCGCC
KspI 131 CCGCGG CCGC^GG
418 CCGCGG
MaeI 28 CTAG C^TAG
439 CTAG
NarI 31 GGCGCC GG^CGCC
148 GGCGCC
PaeR7I 198 CTCGAG C^TCGAG
469 CTCGAG
SacII 131 CCGCGG CCGC^GG
418 CCGCGG
SfoI 31 GGCGCC GGC^GCC
148 GGCGCC
SmaI 36 CCCGGG CCC^GGG
205 CCCGGG
XhoI 198 CTCGAG C^TCGAG
469 CTCGAG
XmaCI 36 CCCGGG C^CCGGG
205 CCCGGG
XmaI 36 CCCGGG C^CCGGG
205 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AvaI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssHII
BssKI BstF5I BstUI Cac8I CfoI
Cfr13I DsaI EaeI FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MaeII MnlI MspA1I MspI MvnI
MwoI NciI NlaIV PstI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI TthHB8I