Restriction map for
pF1KB6560
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BpmI BpuAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tsp509I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 624 CGCG CG^CG
AlwNI 1013 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 778 CCTCAGG CC^T[ACGT]AGG
Aor51HI 112 AGCGCT AGC^GCT
ApaI 396 GGGCCC GGGCC^C
AspI 426 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 347 TGATCA T^GATCA
BlpI 901 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 901 GCTGAGC GC^T[ACGT]AGC
BseRI 849 GAGGAG GAGGAG(10/8)
BsiEI 391 CGGCCG CG[GA][CT]^CG
Bsp1407I 185 TGTACA T^GTACA
BsrGI 185 TGTACA T^GTACA
BsrI 835 ACTGG ACTGG(1/-1)
BstF5I 24 CATCC GGATG(2/0)
BstUI 624 CGCG CG^CG
BstXI 110 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 778 CCTCAGG CC^T[ACGT]AGG
CelII 901 GCTGAGC GC^T[ACGT]AGC
CfoI 113 GCGC GCG^C
DraIII 315 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 391 CGGCCG [CT]^GGCC[GA]
EagI 391 CGGCCG C^GGCCG
EarI 143 CTCTTC CTCTTC(1/4)
EclXI 391 CGGCCG C^GGCCG
Eco47III 112 AGCGCT AGC^GCT
Eco52I 391 CGGCCG C^GGCCG
Eco81I 778 CCTCAGG CC^T[ACGT]AGG
EcoNI 124 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 597 CCATGG C^C[AT][AT]GG
FbaI 347 TGATCA T^GATCA
FokI 24 CATCC GGATG(9/13)
HaeII 112 AGCGCT [GA]GCGC^[CT]
HgaI 622 GACGC GACGC(5/10)
HhaI 113 GCGC GCG^C
HinP1I 113 GCGC G^CGC
HindIII 693 AAGCTT A^AGCTT
Ksp632I 143 CTCTTC CTCTTC(1/4)
MseI 170 TTAA T^TAA
MslI 70 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1016 CAGCTG C[AC]G^C[GT]G
MvnI 624 CGCG CG^CG
MwoI 440 GCTCCCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 597 CCATGG C^CATGG
NspI 31 GCATGT [GA]CATG^[CT]
PflFI 426 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1016 CAGCTG CAG^CTG
SmlI 125 CTCAAG C^T[CT][GA]AG
SspBI 185 TGTACA T^GTACA
SspI 228 AATATT AAT^ATT
StuI 944 AGGCCT AGG^CCT
StyI 597 CCATGG C^C[AT][AT]GG
TaqI 291 TCGA T^CGA
TfiI 1022 GAATC G^A[AT]TC
Tru9I 170 TTAA T^TAA
Tth111I 426 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 291 TCGA T^CGA
XcmI 332 CCAGCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 304 CCGC CCGC(-3/-1)
733 GCGG
AfaI 186 GTAC GT^AC
1009 GTAC
AflIII 69 ACACGT A^C[GA][CT]GT
135 ACACGT
Asp700I 237 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
656 GAAACCCTTC
BanI 120 GGTGCC G^G[CT][GA]CC
327 GGCACC
BbrPI 70 CACGTG CAC^GTG
136 CACGTG
BcnI 389 CCCGG CC^[GC]GG
394 CCGGG
BfaI 665 CTAG C^TAG
962 CTAG
BsaAI 70 CACGTG [CT]AC^GT[GA]
136 CACGTG
BsaI 571 GAGACC GGTCTC(1/5)
858 GAGACC
BstYI 601 GGATCT [GA]^GATC[CT]
955 GGATCT
FauI 303 CCCGC CCCGC(4/6)
733 GCGGG
HapII 390 CCGG C^CGG
394 CCGG
HpaII 390 CCGG C^CGG
394 CCGG
MaeI 665 CTAG C^TAG
962 CTAG
MflI 601 GGATCT [GA]^GATC[CT]
955 GGATCT
MspI 390 CCGG C^CGG
394 CCGG
NciI 389 CCCGG CC^[GC]GG
394 CCGGG
PflMI 314 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAGCAGCTGG
PleI 141 GACTC GAGTC(4/5)
760 GACTC
PmaCI 70 CACGTG CAC^GTG
136 CACGTG
PmlI 70 CACGTG CAC^GTG
136 CACGTG
PstI 435 CTGCAG CTGCA^G
506 CTGCAG
RsaI 186 GTAC GT^AC
1009 GTAC
SacI 214 GAGCTC GAGCT^C
447 GAGCTC
Van91I 314 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAGCAGCTGG
XhoII 601 GGATCT [GA]^GATC[CT]
955 GGATCT
XmnI 237 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
656 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AlwI AspHI AvaII BanII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MvaI
NdeII NlaIII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI