Restriction map for
pF1KE9668
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AflII AflIII AhdI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbrPI BclI BfrI
BglII BlnI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI Eam1105I EcoNI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1261 GACGTC GACGT^C
Acc65I 585 GGTACC G^GTACC
AccI 78 GTCTAC GT^[AC][GT]AC
AgeI 3166 ACCGGT A^CCGGT
AocI 1381 CCTGAGG CC^T[ACGT]AGG
Asp700I 2085 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 585 GGTACC G^GTACC
AspI 433 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1319 TGGCCA TGG^CCA
BbeI 3842 GGCGCC GGCGC^C
BcgI 1566 CGAGCAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 374 GTATCC GTATCC(6/5)
BsaAI 1791 CACGTA [CT]AC^GT[GA]
BsaBI 1953 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3166 ACCGGT [AT]^CCGG[AT]
BsmI 360 GAATGC GAATGC(1/-1)
BstBI 1348 TTCGAA TT^CGAA
BstEII 3169 GGTCACC G^GT[ACGT]ACC
BstPI 3169 GGTCACC G^GT[ACGT]ACC
BstYI 3341 AGATCC [GA]^GATC[CT]
Bsu36I 1381 CCTGAGG CC^T[ACGT]AGG
Eco81I 1381 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3169 GGTCACC G^GT[ACGT]ACC
EcoRI 1935 GAATTC G^AATTC
KasI 3842 GGCGCC G^GCGCC
KpnI 585 GGTACC GGTAC^C
MamI 1953 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3341 AGATCC [GA]^GATC[CT]
MluNI 1319 TGGCCA TGG^CCA
MscI 1319 TGGCCA TGG^CCA
NarI 3842 GGCGCC GG^CGCC
NspV 1348 TTCGAA TT^CGAA
PflFI 433 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3166 ACCGGT A^CCGGT
PpuMI 3575 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 705 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 3813 GGCCCGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3842 GGCGCC GGC^GCC
SfuI 1348 TTCGAA TT^CGAA
SmaI 2191 CCCGGG CCC^GGG
SspI 4065 AATATT AAT^ATT
StuI 3475 AGGCCT AGG^CCT
TfiI 639 GAATC G^A[AT]TC
Tth111I 433 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1427 CCACCAAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3341 AGATCC [GA]^GATC[CT]
XmaCI 2191 CCCGGG C^CCGGG
XmaI 2191 CCCGGG C^CCGGG
XmnI 2085 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1935 GAATTC [GA]^AATT[CT]
4041 GAATTT
AlwI 3342 GATCC GGATC(4/5)
3483 GATCC
Aor51HI 335 AGCGCT AGC^GCT
2003 AGCGCT
ApaI 3518 GGGCCC GGGCC^C
3821 GGGCCC
ApoI 1935 GAATTC [GA]^AATT[CT]
4041 GAATTT
AvaI 2191 CCCGGG C^[CT]CG[GA]G
3692 CTCGGG
BbvCI 631 CCTCAGC CCTCAGC(-5/-2)
3106 CCTCAGC
BfaI 61 CTAG C^TAG
3613 CTAG
BlpI 1534 GCTGAGC GC^T[ACGT]AGC
1851 GCTCAGC
Bpu1102I 1534 GCTGAGC GC^T[ACGT]AGC
1851 GCTCAGC
BsiEI 3388 CGGCCG CG[GA][CT]^CG
3445 CGGCCG
BsoBI 2191 CCCGGG C^[CT]CG[GA]G
3692 CTCGGG
BspHI 512 TCATGA T^CATGA
713 TCATGA
BsrDI 2400 CATTGC GCAATG(2/0)
1742 GCAATG
BstXI 944 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1430 CCAAGGTTGTGG
CelII 1534 GCTGAGC GC^T[ACGT]AGC
1851 GCTCAGC
DraIII 1988 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2560 CACGCAGTG
DrdI 691 GACCTCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3237 GACCCACCAGTC
EagI 3388 CGGCCG C^GGCCG
3445 CGGCCG
EclXI 3388 CGGCCG C^GGCCG
3445 CGGCCG
Eco47III 335 AGCGCT AGC^GCT
2003 AGCGCT
Eco52I 3388 CGGCCG C^GGCCG
3445 CGGCCG
MaeI 61 CTAG C^TAG
3613 CTAG
MseI 302 TTAA T^TAA
442 TTAA
NaeI 974 GCCGGC GCC^GGC
2068 GCCGGC
NgoMIV 974 GCCGGC G^CCGGC
2068 GCCGGC
PflMI 1308 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1524 CCAGCGGCTGG
RcaI 512 TCATGA T^CATGA
713 TCATGA
Tru9I 302 TTAA T^TAA
442 TTAA
Van91I 1308 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1524 CCAGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaLI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I