Restriction map for
pF1KB6131
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI MamI MfeI MroI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 65 GGCGTC G[GA]^CG[CT]C
AgeI 1328 ACCGGT A^CCGGT
ApaI 102 GGGCCC GGGCC^C
AspHI 1248 GTGCTC G[AT]GC[AT]^C
BalI 212 TGGCCA TGG^CCA
BbsI 343 GAAGAC GAAGAC(2/6)
BcgI 726 CGACCTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1388 GGATAC GTATCC(6/5)
BglI 156 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 744 AGATCT A^GATCT
BlpI 498 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 498 GCTCAGC GC^T[ACGT]AGC
BpuAI 343 GAAGAC GAAGAC(2/6)
BsaAI 1156 TACGTG [CT]AC^GT[GA]
BsaHI 65 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1248 GTGCTC G[AT]GC[AT]^C
BsmBI 67 CGTCTC CGTCTC(1/5)
BsrBI 264 GAGCGG CCGCTC(-3/-3)
BstEII 567 GGTGACC G^GT[ACGT]ACC
BstPI 567 GGTGACC G^GT[ACGT]ACC
BstXI 449 CCATAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 498 GCTCAGC GC^T[ACGT]AGC
DsaI 284 CCGCGG C^C[GA][CT]GG
EcoNI 531 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 567 GGTGACC G^GT[ACGT]ACC
EcoT14I 170 CCAAGG C^C[AT][AT]GG
Hin1I 65 GGCGTC G[GA]^CG[CT]C
HincII 1195 GTCAAC GT[CT]^[GA]AC
HindII 1195 GTCAAC GT[CT]^[GA]AC
KspI 284 CCGCGG CCGC^GG
MluI 774 ACGCGT A^CGCGT
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
PflMI 1413 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1328 ACCGGT A^CCGGT
PshAI 1456 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 284 CCGCGG CCGC^GG
SgrAI 1381 CACCGGCG C[GA]^CCGG[CT]G
SmlI 331 CTCAAG C^T[CT][GA]AG
StyI 170 CCAAGG C^C[AT][AT]GG
TaqI 998 TCGA T^CGA
TfiI 180 GATTC G^A[AT]TC
TthHB8I 998 TCGA T^CGA
Van91I 1413 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1466 CCAAAGAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 1047 GTAC GT^AC
1608 GTAC
AflIII 774 ACGCGT A^C[GA][CT]GT
1157 ACGTGT
BsaWI 1106 ACCGGA [AT]^CCGG[AT]
1328 ACCGGT
EaeI 212 TGGCCA [CT]^GGCC[GA]
281 TGGCCG
MaeII 876 ACGT A^CGT
1157 ACGT
MaeIII 568 GTGAC ^GT[ACGT]AC
991 GTGAC
PvuII 397 CAGCTG CAG^CTG
944 CAGCTG
RsaI 1047 GTAC GT^AC
1608 GTAC
SapI 1057 GAAGAGC GCTCTTC(1/4)
1347 GAAGAGC
StuI 905 AGGCCT AGG^CCT
1526 AGGCCT
Tsp45I 568 GTGAC ^GT[GC]AC
991 GTGAC
XbaI 651 TCTAGA T^CTAGA
966 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
Asp700I AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TseI TspRI XhoII XmaCI
XmaI XmnI