Restriction map for
pF1KE1077
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI BalI
BamHI BbeI BbsI BbvCI BcgI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaBI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FseI HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 288 GAATTT [GA]^AATT[CT]
AcyI 220 GGCGTC G[GA]^CG[CT]C
AgeI 596 ACCGGT A^CCGGT
AlwI 306 GATCC GGATC(4/5)
ApoI 288 GAATTT [GA]^AATT[CT]
Asp700I 288 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 238 CTCGAG C^[CT]CG[GA]G
AvaII 311 GGTCC G^G[AT]CC
AviII 33 TGCGCA TGC^GCA
AvrII 69 CCTAGG C^CTAGG
BanII 235 GAGCTC G[GA]GC[CT]^C
BciVI 498 GGATAC GTATCC(6/5)
BclI 453 TGATCA T^GATCA
BfaI 70 CTAG C^TAG
BlnI 69 CCTAGG C^CTAGG
BsaHI 220 GGCGTC G[GA]^CG[CT]C
BsaI 545 GAGACC GGTCTC(1/5)
BsoBI 238 CTCGAG C^[CT]CG[GA]G
BsrBI 241 GAGCGG CCGCTC(-3/-3)
BstXI 503 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 305 AGATCC [GA]^GATC[CT]
EcoT22I 380 ATGCAT ATGCA^T
FbaI 453 TGATCA T^GATCA
FspI 33 TGCGCA TGC^GCA
Hin1I 220 GGCGTC G[GA]^CG[CT]C
HinfI 651 GAATC G^A[ACGT]TC
MaeI 70 CTAG C^TAG
MaeIII 413 GTTAC ^GT[ACGT]AC
MflI 305 AGATCC [GA]^GATC[CT]
MseI 374 TTAA T^TAA
MslI 612 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 273 CCATGG C^CATGG
NsiI 380 ATGCAT ATGCA^T
NspI 151 GCATGC [GA]CATG^[CT]
PaeR7I 238 CTCGAG C^TCGAG
PinAI 596 ACCGGT A^CCGGT
PstI 157 CTGCAG CTGCA^G
PvuII 37 CAGCTG CAG^CTG
SacI 235 GAGCTC GAGCT^C
SbfI 156 CCTGCAGG CCTGCA^GG
SfcI 157 CTGCAG C^T[GA][CT]AG
SmlI 238 CTCGAG C^T[CT][GA]AG
SphI 151 GCATGC GCATG^C
Sse8387I 156 CCTGCAGG CCTGCA^GG
TfiI 651 GAATC G^A[AT]TC
Tru9I 374 TTAA T^TAA
TspRI 475 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 238 CTCGAG C^TCGAG
XhoII 305 AGATCC [GA]^GATC[CT]
XmnI 288 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 105 GTAC GT^AC
118 GTAC
AflIII 82 ACGTGT A^C[GA][CT]GT
135 ACACGT
AluI 38 AGCT AG^CT
236 AGCT
Aor51HI 205 AGCGCT AGC^GCT
665 AGCGCT
BbrPI 81 CACGTG CAC^GTG
316 CACGTG
BsaAI 81 CACGTG [CT]AC^GT[GA]
316 CACGTG
BsaWI 308 TCCGGT [AT]^CCGG[AT]
596 ACCGGT
BsmAI 262 GAGAC GTCTC(1/5)
545 GAGAC
BssKI 217 CCTGG ^CC[ACGT]GG
539 CCTGG
BstF5I 582 CATCC GGATG(2/0)
378 GGATG
BstNI 217 CCTGG CC^[AT]GG
539 CCTGG
Cfr13I 46 GGGCC G^G[ACGT]CC
311 GGTCC
DdeI 96 CTTAG C^T[ACGT]AG
440 CTGAG
DsaI 230 CCGTGG C^C[GA][CT]GG
273 CCATGG
EarI 386 CTCTTC CTCTTC(1/4)
637 GAAGAG
Eco47III 205 AGCGCT AGC^GCT
665 AGCGCT
EcoRII 217 CCTGG ^CC[AT]GG
539 CCTGG
FokI 582 CATCC GGATG(9/13)
378 GGATG
HaeIII 47 GGCC GG^CC
366 GGCC
HgaI 74 GACGC GACGC(5/10)
221 GCGTC
Ksp632I 386 CTCTTC CTCTTC(1/4)
637 GAAGAG
MspA1I 13 CAGCGG C[AC]G^C[GT]G
37 CAGCTG
MvaI 217 CCTGG CC^[AT]GG
539 CCTGG
NaeI 48 GCCGGC GCC^GGC
590 GCCGGC
NgoMIV 48 GCCGGC G^CCGGC
590 GCCGGC
PmaCI 81 CACGTG CAC^GTG
316 CACGTG
PmlI 81 CACGTG CAC^GTG
316 CACGTG
RsaI 105 GTAC GT^AC
118 GTAC
Sau96I 46 GGGCC G^G[ACGT]CC
311 GGTCC
ScrFI 217 CCTGG CC^[ACGT]GG
539 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AspHI BanI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI Cac8I CfoI Cfr10I
DpnII EcoT14I FauI Fnu4HI HaeII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MnlI MspI
MwoI NdeII NlaIII NlaIV Sau3AI
SfaNI StyI TaqI TseI Tsp509I
TthHB8I