Restriction map for
pF1KE1012
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI DpnII DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MamI MboI MfeI MflI MluI
MroI MseI MslI MunI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI Sau3AI
ScaI SfiI SfoI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 181 AAATTC [GA]^AATT[CT]
ApaI 53 GGGCCC GGGCC^C
ApoI 181 AAATTC [GA]^AATT[CT]
AspHI 699 GAGCAC G[AT]GC[AT]^C
BalI 731 TGGCCA TGG^CCA
BanI 593 GGCACC G^G[CT][GA]CC
BbsI 307 GTCTTC GAAGAC(2/6)
BpuAI 307 GTCTTC GAAGAC(2/6)
BsiHKAI 699 GAGCAC G[AT]GC[AT]^C
BsmBI 107 GAGACG CGTCTC(1/5)
BsrBI 88 GAGCGG CCGCTC(-3/-3)
CpoI 293 CGGACCG CG^G[AT]CCG
DrdI 670 GACCAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 631 CTCTTC CTCTTC(1/4)
EcoT14I 761 CCAAGG C^C[AT][AT]GG
FseI 315 GGCCGGCC GGCCGG^CC
HgaI 154 GACGC GACGC(5/10)
Ksp632I 631 CTCTTC CTCTTC(1/4)
KspI 273 CCGCGG CCGC^GG
MaeII 818 ACGT A^CGT
MluNI 731 TGGCCA TGG^CCA
MscI 731 TGGCCA TGG^CCA
PflMI 723 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 293 CGGACCG CG^G[AT]CCG
SacII 273 CCGCGG CCGC^GG
SapI 630 GCTCTTC GCTCTTC(1/4)
SbfI 651 CCTGCAGG CCTGCA^GG
SgrAI 638 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 651 CCTGCAGG CCTGCA^GG
StyI 761 CCAAGG C^C[AT][AT]GG
TaqI 750 TCGA T^CGA
Tsp509I 182 AATT ^AATT
TthHB8I 750 TCGA T^CGA
Van91I 723 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 413 CCAACCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 385 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
510 CAGGTACTG
BanII 53 GGGCCC G[GA]GC[CT]^C
348 GAGCCC
BsaI 532 GAGACC GGTCTC(1/5)
426 GGTCTC
BseRI 345 GAGGAG GAGGAG(10/8)
685 GAGGAG
BsgI 694 GTGCAG GTGCAG(16/14)
820 GTGCAG
BsiEI 95 CGGCCG CG[GA][CT]^CG
314 CGGCCG
BspMI 654 GCAGGT ACCTGC(4/8)
690 GCAGGT
BssHII 31 GCGCGC G^CGCGC
113 GCGCGC
BstXI 357 CCACTACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
788 CCAGCCGCCTGG
DdeI 208 CTGAG C^T[ACGT]AG
879 CTTAG
DraII 52 GGGGCCC [GA]G^G[ACGT]CC[CT]
647 AGGCCCT
DsaI 273 CCGCGG C^C[GA][CT]GG
782 CCACGG
EagI 95 CGGCCG C^GGCCG
314 CGGCCG
EclXI 95 CGGCCG C^GGCCG
314 CGGCCG
Eco52I 95 CGGCCG C^GGCCG
314 CGGCCG
EcoO109I 52 GGGGCCC [GA]G^G[ACGT]CC[CT]
647 AGGCCCT
HaeII 3 GGCGCT [GA]GCGC^[CT]
119 GGCGCT
MaeIII 482 GTGAC ^GT[ACGT]AC
799 GTCAC
NlaIII 745 CATG CATG^
768 CATG
SexAI 416 ACCTGGT A^CC[AT]GGT
671 ACCAGGT
SmaI 63 CCCGGG CCC^GGG
300 CCCGGG
Tsp45I 482 GTGAC ^GT[GC]AC
799 GTCAC
XmaCI 63 CCCGGG C^CCGGG
300 CCCGGG
XmaI 63 CCCGGG C^CCGGG
300 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIV
PleI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI TseI TspRI