Restriction map for
pF1KB3024
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AclI AflII AgeI AocI AscI
AseI AsnI Asp700I AviII AvrII
BbrPI BciVI BfrI BlnI BlpI
Bpu1102I BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bsu36I CelII ClaI CpoI DraI
EagI EclXI Eco52I Eco81I EcoT22I
FseI FspI HindIII HpaI MfeI
MluI MseI MunI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SfiI SnaBI SpeI
SspBI SspI SwaI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2244 GACGTC GACGT^C
Acc65I 3363 GGTACC G^GTACC
AccIII 2336 TCCGGA T^CCGGA
AflIII 1121 ACACGT A^C[GA][CT]GT
AhdI 1179 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3242 AGCGCT AGC^GCT
Asp718I 3363 GGTACC G^GTACC
AspEI 1179 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2713 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1561 GTCTTC GAAGAC(2/6)
BcgI 1840 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3149 TGATCA T^GATCA
BpuAI 1561 GTCTTC GAAGAC(2/6)
BseAI 2336 TCCGGA T^CCGGA
BsmBI 2440 GAGACG CGTCTC(1/5)
BspEI 2336 TCCGGA T^CCGGA
Bst1107I 1155 GTATAC GTA^TAC
BstBI 716 TTCGAA TT^CGAA
BstZ17I 1155 GTATAC GTA^TAC
DrdI 1552 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1179 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3242 AGCGCT AGC^GCT
EcoRV 517 GATATC GAT^ATC
FbaI 3149 TGATCA T^GATCA
HincII 1876 GTCAAC GT[CT]^[GA]AC
HindII 1876 GTCAAC GT[CT]^[GA]AC
KpnI 3363 GGTACC GGTAC^C
KspI 2730 CCGCGG CCGC^GG
MroI 2336 TCCGGA T^CCGGA
NdeI 2630 CATATG CA^TATG
NspV 716 TTCGAA TT^CGAA
PflFI 2713 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2730 CCGCGG CCGC^GG
SbfI 510 CCTGCAGG CCTGCA^GG
SexAI 3338 ACCTGGT A^CC[AT]GGT
SfuI 716 TTCGAA TT^CGAA
SgrAI 2977 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 78 CTTGAG C^T[CT][GA]AG
SphI 2155 GCATGC GCATG^C
Sse8387I 510 CCTGCAGG CCTGCA^GG
StuI 696 AGGCCT AGG^CCT
Tth111I 2713 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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Alw44I 366 GTGCAC G^TGCAC
1720 GTGCAC
ApaLI 366 GTGCAC G^TGCAC
1720 GTGCAC
BalI 2540 TGGCCA TGG^CCA
3227 TGGCCA
BbeI 1165 GGCGCC GGCGC^C
1606 GGCGCC
BglI 148 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2642 GCCTTCTGGGC
BglII 731 AGATCT A^GATCT
1397 AGATCT
BsaAI 538 TACGTG [CT]AC^GT[GA]
2605 TACGTG
BsaBI 2307 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2424 GATGCGGATC
DraIII 1723 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1866 CACCCAGTG
EarI 1501 GAAGAG CTCTTC(1/4)
3490 GAAGAG
EcoNI 435 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
673 CCTGTCACAGG
EcoRI 1592 GAATTC G^AATTC
3214 GAATTC
KasI 1165 GGCGCC G^GCGCC
1606 GGCGCC
Ksp632I 1501 GAAGAG CTCTTC(1/4)
3490 GAAGAG
MamI 2307 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2424 GATGCGGATC
MluNI 2540 TGGCCA TGG^CCA
3227 TGGCCA
MscI 2540 TGGCCA TGG^CCA
3227 TGGCCA
NaeI 1015 GCCGGC GCC^GGC
2978 GCCGGC
NarI 1165 GGCGCC GG^CGCC
1606 GGCGCC
NcoI 264 CCATGG C^CATGG
290 CCATGG
NgoMIV 1015 GCCGGC G^CCGGC
2978 GCCGGC
PflMI 2037 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2945 CCATCAAGTGG
ScaI 1926 AGTACT AGT^ACT
3875 AGTACT
SfoI 1165 GGCGCC GGC^GCC
1606 GGCGCC
TaqI 717 TCGA T^CGA
3941 TCGA
TfiI 113 GATTC G^A[AT]TC
3194 GATTC
TthHB8I 717 TCGA T^CGA
3941 TCGA
Van91I 2037 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2945 CCATCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmaCI
XmaI