Restriction map for
pF1KB9268
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 1432 GAATTT [GA]^AATT[CT]
AflIII 81 ACATGT A^C[GA][CT]GT
AhdI 1106 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 922 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 592 CCTGAGG CC^T[ACGT]AGG
ApoI 1432 GAATTT [GA]^AATT[CT]
AspEI 1106 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1660 GAGCTC G[AT]GC[AT]^C
AvaII 1123 GGTCC G^G[AT]CC
BalI 491 TGGCCA TGG^CCA
BanII 1660 GAGCTC G[GA]GC[CT]^C
BcgI 831 CGAAGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 569 GGATAC GTATCC(6/5)
BfaI 1429 CTAG C^TAG
BsaBI 690 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1693 GAGACC GGTCTC(1/5)
BsaWI 192 TCCGGT [AT]^CCGG[AT]
BsgI 1580 CTGCAC GTGCAG(16/14)
BsiHKAI 1660 GAGCTC G[AT]GC[AT]^C
BsmBI 900 GAGACG CGTCTC(1/5)
BsmFI 1124 GTCCC GGGAC(10/14)
BspLU11I 81 ACATGT A^CATGT
BstYI 19 GGATCT [GA]^GATC[CT]
Bsu36I 592 CCTGAGG CC^T[ACGT]AGG
Cfr13I 1123 GGTCC G^G[ACGT]CC
Eam1105I 1106 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 592 CCTGAGG CC^T[ACGT]AGG
MaeI 1429 CTAG C^TAG
MaeII 1049 ACGT A^CGT
MamI 690 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 19 GGATCT [GA]^GATC[CT]
MluNI 491 TGGCCA TGG^CCA
MscI 491 TGGCCA TGG^CCA
NcoI 207 CCATGG C^CATGG
NspI 81 ACATGT [GA]CATG^[CT]
PshAI 31 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1660 GAGCTC GAGCT^C
SapI 241 GAAGAGC GCTCTTC(1/4)
Sau96I 1123 GGTCC G^G[ACGT]CC
SspI 912 AATATT AAT^ATT
StuI 589 AGGCCT AGG^CCT
XcmI 1227 CCAGGAGAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 19 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 214 GCGG CCGC(-3/-1)
217 GCGG
AlwI 861 GATCC GGATC(4/5)
19 GGATC
Asp700I 241 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGATTTTC
BanI 195 GGTGCC G^G[CT][GA]CC
1704 GGTGCC
BbsI 97 GAAGAC GAAGAC(2/6)
400 GAAGAC
BclI 1029 TGATCA T^GATCA
1166 TGATCA
BcnI 476 CCGGG CC^[GC]GG
652 CCCGG
BpuAI 97 GAAGAC GAAGAC(2/6)
400 GAAGAC
Bsp1407I 327 TGTACA T^GTACA
1284 TGTACA
BspHI 159 TCATGA T^CATGA
500 TCATGA
BsrGI 327 TGTACA T^GTACA
1284 TGTACA
BstF5I 465 GGATG GGATG(2/0)
1392 GGATG
BstXI 1179 CCAGCACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1708 CCAGTGAGCTGG
DsaI 207 CCATGG C^C[GA][CT]GG
1127 CCGTGG
EarI 241 GAAGAG CTCTTC(1/4)
1023 GAAGAG
FbaI 1029 TGATCA T^GATCA
1166 TGATCA
FokI 465 GGATG GGATG(9/13)
1392 GGATG
HincII 12 GTCAAC GT[CT]^[GA]AC
562 GTCAAC
HindII 12 GTCAAC GT[CT]^[GA]AC
562 GTCAAC
HphI 127 GGTGA GGTGA(8/7)
544 GGTGA
Ksp632I 241 GAAGAG CTCTTC(1/4)
1023 GAAGAG
MslI 172 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
538 CATGTTGGTG
MspA1I 70 CAGCTG C[AC]G^C[GT]G
1043 CAGCTG
NciI 476 CCGGG CC^[GC]GG
652 CCCGG
PstI 73 CTGCAG CTGCA^G
515 CTGCAG
PvuII 70 CAGCTG CAG^CTG
1043 CAGCTG
RcaI 159 TCATGA T^CATGA
500 TCATGA
SfaNI 805 GATGC GCATC(5/9)
838 GATGC
SfcI 73 CTGCAG C^T[GA][CT]AG
515 CTGCAG
SspBI 327 TGTACA T^GTACA
1284 TGTACA
TfiI 156 GATTC G^A[AT]TC
497 GAATC
XmnI 241 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmI
Bsp1286I BsrI BssKI BstNI Cac8I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI StyI Tru9I TseI Tsp45I
Tsp509I TspRI