Restriction map for
pF1KB3539
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 591 GGTACC G^GTACC
AlwNI 451 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 343 CCTCAGG CC^T[ACGT]AGG
Asp700I 738 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 591 GGTACC G^GTACC
AspHI 802 GAGCTC G[AT]GC[AT]^C
AvaI 595 CCCGAG C^[CT]CG[GA]G
AvaII 585 GGACC G^G[AT]CC
BanII 802 GAGCTC G[GA]GC[CT]^C
BcnI 824 CCCGG CC^[GC]GG
BglII 282 AGATCT A^GATCT
BmyI 802 GAGCTC G[AGT]GC[ACT]^C
BpmI 933 CTGGAG CTGGAG(16/14)
BsaWI 389 ACCGGA [AT]^CCGG[AT]
BseRI 625 GAGGAG GAGGAG(10/8)
BsiHKAI 802 GAGCTC G[AT]GC[AT]^C
BsmFI 560 GGGAC GGGAC(10/14)
BsoBI 595 CCCGAG C^[CT]CG[GA]G
Bsp1286I 802 GAGCTC G[AGT]GC[ACT]^C
BspMI 416 ACCTGC ACCTGC(4/8)
BsrDI 881 GCAATG GCAATG(2/0)
BsrFI 332 GCCGGT [GA]^CCGG[CT]
BssSI 926 CACGAG CACGAG(-5/-1)
BstXI 635 CCAAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 282 AGATCT [GA]^GATC[CT]
Bsu36I 343 CCTCAGG CC^T[ACGT]AGG
Cac8I 263 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 332 GCCGGT [GA]^CCGG[CT]
DpnII 283 GATC ^GATC
EarI 295 GAAGAG CTCTTC(1/4)
Eco81I 343 CCTCAGG CC^T[ACGT]AGG
HincII 54 GTTGAC GT[CT]^[GA]AC
HindII 54 GTTGAC GT[CT]^[GA]AC
HindIII 356 AAGCTT A^AGCTT
KpnI 591 GGTACC GGTAC^C
Ksp632I 295 GAAGAG CTCTTC(1/4)
MboI 283 GATC ^GATC
MflI 282 AGATCT [GA]^GATC[CT]
MslI 1005 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 454 CAGCTG C[AC]G^C[GT]G
NciI 824 CCCGG CC^[GC]GG
NdeII 283 GATC ^GATC
NlaIII 141 CATG CATG^
PflMI 576 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 442 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
SacI 802 GAGCTC GAGCT^C
Sau3AI 283 GATC ^GATC
SexAI 587 ACCTGGT A^CC[AT]GGT
SfcI 442 CTGCAG C^T[GA][CT]AG
SmlI 544 CTCAAG C^T[CT][GA]AG
TaqI 507 TCGA T^CGA
Tsp45I 924 GTCAC ^GT[GC]AC
TthHB8I 507 TCGA T^CGA
Van91I 576 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 534 TCTAGA T^CTAGA
XcmI 635 CCAAGCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 282 AGATCT [GA]^GATC[CT]
XmnI 738 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 34 AAATTC [GA]^AATT[CT]
818 AAATTT
ApoI 34 AAATTC [GA]^AATT[CT]
818 AAATTT
BanI 106 GGTGCC G^G[CT][GA]CC
591 GGTACC
BbsI 775 GAAGAC GAAGAC(2/6)
859 GTCTTC
BbvI 450 GCAGC GCAGC(8/12)
453 GCAGC
BfaI 535 CTAG C^TAG
869 CTAG
BpuAI 775 GAAGAC GAAGAC(2/6)
859 GTCTTC
BsmAI 872 GAGAC GTCTC(1/5)
240 GTCTC
BstNI 483 CCAGG CC^[AT]GG
588 CCTGG
DraIII 577 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
916 CACCAAGTG
EcoRII 483 CCAGG ^CC[AT]GG
588 CCTGG
Fnu4HI 450 GCAGC GC^[ACGT]GC
453 GCAGC
HphI 735 GGTGA GGTGA(8/7)
888 GGTGA
ItaI 450 GCAGC GC^[ACGT]GC
453 GCAGC
MaeI 535 CTAG C^TAG
869 CTAG
MaeII 473 ACGT A^CGT
678 ACGT
MseI 503 TTAA T^TAA
608 TTAA
MvaI 483 CCAGG CC^[AT]GG
588 CCTGG
PleI 791 GACTC GAGTC(4/5)
929 GAGTC
TfiI 120 GATTC G^A[AT]TC
743 GATTC
Tru9I 503 TTAA T^TAA
608 TTAA
TseI 450 GCAGC G^C[AT]GC
453 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BsiYI BslI BsrI
BssKI BstF5I Cfr13I DdeI FokI
HaeIII HapII HinfI HpaII MaeIII
MboII MnlI MspI MwoI NlaIV
RsaI Sau96I ScrFI SfaNI Tsp509I
TspRI