Restriction map for
pF1KB3509
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AhdI 374 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 592 GTGCAC G^TGCAC
ApaI 7 GGGCCC GGGCC^C
ApaLI 592 GTGCAC G^TGCAC
AspEI 374 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 592 GTGCAC G[AT]GC[AT]^C
AvaII 217 GGACC G^G[AT]CC
AviII 341 TGCGCA TGC^GCA
BanII 7 GGGCCC G[GA]GC[CT]^C
BbsI 297 GAAGAC GAAGAC(2/6)
BcnI 560 CCCGG CC^[GC]GG
BpuAI 297 GAAGAC GAAGAC(2/6)
BsaWI 572 ACCGGA [AT]^CCGG[AT]
BseRI 190 GAGGAG GAGGAG(10/8)
BsiHKAI 592 GTGCAC G[AT]GC[AT]^C
BsmI 46 GCATTC GAATGC(1/-1)
BsrBI 193 GAGCGG CCGCTC(-3/-3)
BsrFI 313 GCCGGT [GA]^CCGG[CT]
BsrI 82 ACTGG ACTGG(1/-1)
Cfr10I 313 GCCGGT [GA]^CCGG[CT]
DraIII 818 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 260 CCATGG C^C[GA][CT]GG
Eam1105I 374 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 260 CCATGG C^C[AT][AT]GG
FauI 10 CCCGC CCCGC(4/6)
FspI 341 TGCGCA TGC^GCA
MspA1I 140 CAGCTG C[AC]G^C[GT]G
NciI 560 CCCGG CC^[GC]GG
NcoI 260 CCATGG C^CATGG
NspI 471 GCATGT [GA]CATG^[CT]
PflMI 112 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 216 AGGACCT [GA]G^G[AT]CC[CT]
PstI 143 CTGCAG CTGCA^G
PvuII 140 CAGCTG CAG^CTG
SfcI 143 CTGCAG C^T[GA][CT]AG
SspI 421 AATATT AAT^ATT
StyI 260 CCATGG C^C[AT][AT]GG
TfiI 906 GAATC G^A[AT]TC
Van91I 112 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 12 CGCG CG^CG
363 CGCG
AfaI 690 GTAC GT^AC
718 GTAC
BmyI 7 GGGCCC G[AGT]GC[ACT]^C
592 GTGCAC
BpmI 878 CTCCAG CTGGAG(16/14)
221 CTGGAG
BsaI 27 GGTCTC GGTCTC(1/5)
805 GGTCTC
BsaJI 260 CCATGG C^C[ACGT][ACGT]GG
515 CCGAGG
BsgI 493 GTGCAG GTGCAG(16/14)
788 GTGCAG
BsmFI 871 GGGAC GGGAC(10/14)
954 GGGAC
Bsp1286I 7 GGGCCC G[AGT]GC[ACT]^C
592 GTGCAC
BstEII 66 GGTGACC G^GT[ACGT]ACC
316 GGTCACC
BstNI 112 CCAGG CC^[AT]GG
220 CCTGG
BstPI 66 GGTGACC G^GT[ACGT]ACC
316 GGTCACC
BstUI 12 CGCG CG^CG
363 CGCG
BstYI 17 GGATCT [GA]^GATC[CT]
723 AGATCC
DraII 216 AGGACCT [GA]G^G[ACGT]CC[CT]
288 AGGCCCT
EcoO109I 216 AGGACCT [GA]G^G[ACGT]CC[CT]
288 AGGCCCT
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
316 GGTCACC
EcoRII 112 CCAGG ^CC[AT]GG
220 CCTGG
MaeII 200 ACGT A^CGT
581 ACGT
MboII 153 GAAGA GAAGA(8/7)
297 GAAGA
MflI 17 GGATCT [GA]^GATC[CT]
723 AGATCC
MslI 585 CACGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CATGCGGATG
MvaI 112 CCAGG CC^[AT]GG
220 CCTGG
MvnI 12 CGCG CG^CG
363 CGCG
RsaI 690 GTAC GT^AC
718 GTAC
SfaNI 163 GATGC GCATC(5/9)
939 GATGC
TaqI 389 TCGA T^CGA
435 TCGA
TthHB8I 389 TCGA T^CGA
435 TCGA
XhoII 17 GGATCT [GA]^GATC[CT]
723 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BsiYI BslI BsmAI BsrDI
BssKI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MnlI
MseI MspI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI