Restriction map for
pF1KB6010
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BfrI BglI BglII
BlnI BsaAI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI BspDI BspMI
BsrBI BssHII BssSI BstAPI BstEII
BstPI BstUI CfoI ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MluI
MluNI MscI MvnI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 2442 GTATAC GT^[AC][GT]AC
AccIII 1847 TCCGGA T^CCGGA
AflIII 2423 ACATGT A^C[GA][CT]GT
AhdI 3163 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3400 CCTGAGG CC^T[ACGT]AGG
ApaI 144 GGGCCC GGGCC^C
AseI 715 ATTAAT AT^TAAT
AsnI 715 ATTAAT AT^TAAT
AspEI 3163 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1556 GTGCTC G[AT]GC[AT]^C
AspI 1358 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 913 CGAGCTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 591 GGATAC GTATCC(6/5)
BclI 2883 TGATCA T^GATCA
BcnI 3195 CCGGG CC^[GC]GG
BlpI 2475 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2475 GCTCAGC GC^T[ACGT]AGC
BsaWI 1847 TCCGGA [AT]^CCGG[AT]
BseAI 1847 TCCGGA T^CCGGA
BsiHKAI 1556 GTGCTC G[AT]GC[AT]^C
BsmAI 2168 GAGAC GTCTC(1/5)
BsmI 855 GAATGC GAATGC(1/-1)
Bsp1407I 879 TGTACA T^GTACA
BspEI 1847 TCCGGA T^CCGGA
BspLU11I 2423 ACATGT A^CATGT
BsrGI 879 TGTACA T^GTACA
Bst1107I 2442 GTATAC GTA^TAC
BstBI 434 TTCGAA TT^CGAA
BstNI 660 CCTGG CC^[AT]GG
BstXI 222 CCAACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2442 GTATAC GTA^TAC
Bsu36I 3400 CCTGAGG CC^T[ACGT]AGG
CelII 2475 GCTCAGC GC^T[ACGT]AGC
DraII 143 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1517 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1264 CGGCCA [CT]^GGCC[GA]
Eam1105I 3163 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3400 CCTGAGG CC^T[ACGT]AGG
EcoO109I 143 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 660 CCTGG ^CC[AT]GG
FbaI 2883 TGATCA T^GATCA
MfeI 3506 CAATTG C^AATTG
MroI 1847 TCCGGA T^CCGGA
MunI 3506 CAATTG C^AATTG
MvaI 660 CCTGG CC^[AT]GG
NciI 3195 CCGGG CC^[GC]GG
NdeI 412 CATATG CA^TATG
NheI 3144 GCTAGC G^CTAGC
NlaIV 144 GGGCCC GG[ACGT]^[ACGT]CC
NspV 434 TTCGAA TT^CGAA
PflFI 1358 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1516 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 715 ATTAAT AT^TAAT
ScaI 1778 AGTACT AGT^ACT
SfuI 434 TTCGAA TT^CGAA
SspBI 879 TGTACA T^GTACA
StuI 2357 AGGCCT AGG^CCT
Tth111I 1358 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1516 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1404 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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BanII 144 GGGCCC G[GA]GC[CT]^C
3382 GAGCCC
BpmI 11 CTCCAG CTGGAG(16/14)
1283 CTGGAG
BseRI 176 GAGGAG GAGGAG(10/8)
1327 GAGGAG
BsmFI 1454 GGGAC GGGAC(10/14)
1624 GGGAC
BspHI 3074 TCATGA T^CATGA
3413 TCATGA
BsrFI 1262 ACCGGC [GA]^CCGG[CT]
1997 ACCGGC
BssKI 660 CCTGG ^CC[ACGT]GG
3195 CCGGG
Cfr10I 1262 ACCGGC [GA]^CCGG[CT]
1997 ACCGGC
DrdI 625 GACTCACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1031 GACTCAAATGTC
EcoRI 451 GAATTC G^AATTC
529 GAATTC
EcoT14I 485 CCATGG C^C[AT][AT]GG
3578 CCATGG
EcoT22I 1006 ATGCAT ATGCA^T
2048 ATGCAT
MaeII 2333 ACGT A^CGT
2859 ACGT
MspA1I 1280 CAGCTG C[AC]G^C[GT]G
2642 CAGCTG
NcoI 485 CCATGG C^CATGG
3578 CCATGG
NsiI 1006 ATGCAT ATGCA^T
2048 ATGCAT
PvuII 1280 CAGCTG CAG^CTG
2642 CAGCTG
RcaI 3074 TCATGA T^CATGA
3413 TCATGA
ScrFI 660 CCTGG CC^[ACGT]GG
3195 CCGGG
SphI 1425 GCATGC GCATG^C
3260 GCATGC
SspI 861 AATATT AAT^ATT
1852 AATATT
StyI 485 CCATGG C^C[AT][AT]GG
3578 CCATGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaJI
BsgI BsiYI BslI Bsp1286I BsrDI
BsrI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRV Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MwoI NdeII NlaIII
NspI PleI PstI RsaI SapI
Sau3AI Sau96I SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI