Restriction map for
pF1KB9295
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FokI FspI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
MaeII MaeIII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NarI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI Sau3AI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tsp45I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwNI 323 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 315 GAGCTC G[AT]GC[AT]^C
AspI 37 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 148 GGCACC G^G[CT][GA]CC
BanII 315 GAGCTC G[GA]GC[CT]^C
BbsI 43 GTCTTC GAAGAC(2/6)
BbvI 533 GCAGC GCAGC(8/12)
BciVI 129 GTATCC GTATCC(6/5)
BfaI 169 CTAG C^TAG
BmyI 315 GAGCTC G[AGT]GC[ACT]^C
BpuAI 43 GTCTTC GAAGAC(2/6)
BsiHKAI 315 GAGCTC G[AT]GC[AT]^C
BsiYI 596 CCAGTGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 596 CCAGTGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 315 GAGCTC G[AGT]GC[ACT]^C
BsrFI 75 GCCGGC [GA]^CCGG[CT]
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DraII 171 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 10 CCGCGG C^C[GA][CT]GG
EaeI 73 TGGCCG [CT]^GGCC[GA]
EcoO109I 171 AGGTCCC [GA]G^G[ACGT]CC[CT]
FseI 74 GGCCGGCC GGCCGG^CC
HaeII 103 GGCGCT [GA]GCGC^[CT]
HgaI 6 GCGTC GACGC(5/10)
HphI 376 GGTGA GGTGA(8/7)
KspI 10 CCGCGG CCGC^GG
MaeI 169 CTAG C^TAG
MseI 242 TTAA T^TAA
MspA1I 10 CCGCGG C[AC]G^C[GT]G
MwoI 6 GCGTCCGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 75 GCCGGC GCC^GGC
NgoMIV 75 GCCGGC G^CCGGC
NlaIII 154 CATG CATG^
PflFI 37 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 171 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 350 CTGCAG CTGCA^G
SacI 315 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SapI 317 GCTCTTC GCTCTTC(1/4)
SexAI 38 ACCTGGT A^CC[AT]GGT
SfcI 350 CTGCAG C^T[GA][CT]AG
Tru9I 242 TTAA T^TAA
TseI 533 GCAGC G^C[AT]GC
Tsp509I 303 AATT ^AATT
Tth111I 37 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 24 GTAC GT^AC
201 GTAC
AluI 316 AGCT AG^CT
535 AGCT
AvaII 172 GGTCC G^G[AT]CC
605 GGACC
BcnI 80 CCCGG CC^[GC]GG
476 CCCGG
BpmI 116 CTCCAG CTGGAG(16/14)
485 CTCCAG
BsaJI 10 CCGCGG C^C[ACGT][ACGT]GG
273 CCCAGG
CfoI 4 GCGC GCG^C
104 GCGC
DdeI 329 CTGAG C^T[ACGT]AG
539 CTGAG
EarI 318 CTCTTC CTCTTC(1/4)
517 GAAGAG
Fnu4HI 12 GCGGC GC^[ACGT]GC
533 GCAGC
HhaI 4 GCGC GCG^C
104 GCGC
HinP1I 4 GCGC G^CGC
104 GCGC
ItaI 12 GCGGC GC^[ACGT]GC
533 GCAGC
Ksp632I 318 CTCTTC CTCTTC(1/4)
517 GAAGAG
NciI 80 CCCGG CC^[GC]GG
476 CCCGG
RsaI 24 GTAC GT^AC
201 GTAC
SfaNI 355 GATGC GCATC(5/9)
550 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI BsmFI BsrI BssKI
BstNI BstUI Cac8I Cfr13I EcoRII
FauI HaeIII HapII HpaII MboII
MnlI MspI MvaI MvnI NlaIV
Sau96I ScrFI TspRI