Restriction map for
pF1KE1003
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HapII Hin1I HincII HindII HindIII
HinfI HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 216 GTAC GT^AC
BamHI 113 GGATCC G^GATCC
BanI 135 GGTGCC G^G[CT][GA]CC
BanII 227 GAGCCC G[GA]GC[CT]^C
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
BclI 206 TGATCA T^GATCA
BpmI 313 CTGGAG CTGGAG(16/14)
BsaAI 415 TACGTG [CT]AC^GT[GA]
BseRI 126 CTCCTC GAGGAG(10/8)
BsgI 276 CTGCAC GTGCAG(16/14)
BsmAI 241 GAGAC GTCTC(1/5)
Bsp1407I 215 TGTACA T^GTACA
BsrDI 375 CATTGC GCAATG(2/0)
BsrGI 215 TGTACA T^GTACA
BsrI 38 CCAGT ACTGG(1/-1)
BstEII 252 GGTCACC G^GT[ACGT]ACC
BstF5I 210 CATCC GGATG(2/0)
BstPI 252 GGTCACC G^GT[ACGT]ACC
BstYI 113 GGATCC [GA]^GATC[CT]
CfoI 302 GCGC GCG^C
EarI 76 CTCTTC CTCTTC(1/4)
EcoNI 147 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 252 GGTCACC G^GT[ACGT]ACC
EcoT14I 257 CCTTGG C^C[AT][AT]GG
FauI 7 CCCGC CCCGC(4/6)
FbaI 206 TGATCA T^GATCA
FokI 210 CATCC GGATG(9/13)
HaeII 301 AGCGCC [GA]GCGC^[CT]
HgaI 262 GACGC GACGC(5/10)
HhaI 302 GCGC GCG^C
HinP1I 302 GCGC G^CGC
Ksp632I 76 CTCTTC CTCTTC(1/4)
MaeII 416 ACGT A^CGT
MaeIII 253 GTCAC ^GT[ACGT]AC
MflI 113 GGATCC [GA]^GATC[CT]
MseI 439 TTAA T^TAA
RsaI 216 GTAC GT^AC
SapI 75 GCTCTTC GCTCTTC(1/4)
SfaNI 336 GATGC GCATC(5/9)
SfcI 354 CTACAG C^T[GA][CT]AG
SspBI 215 TGTACA T^GTACA
StyI 257 CCTTGG C^C[AT][AT]GG
Tru9I 439 TTAA T^TAA
Tsp45I 253 GTCAC ^GT[GC]AC
Tsp509I 222 AATT ^AATT
XhoII 113 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 114 GATCC GGATC(4/5)
113 GGATC
AvaII 150 GGTCC G^G[AT]CC
420 GGTCC
BalI 191 TGGCCA TGG^CCA
323 TGGCCA
BbsI 52 GTCTTC GAAGAC(2/6)
453 GTCTTC
BmyI 136 GTGCCC G[AGT]GC[ACT]^C
227 GAGCCC
BpuAI 52 GTCTTC GAAGAC(2/6)
453 GTCTTC
Bsp1286I 136 GTGCCC G[AGT]GC[ACT]^C
227 GAGCCC
DdeI 298 CTCAG C^T[ACGT]AG
306 CTCAG
DpnII 114 GATC ^GATC
207 GATC
DraII 101 GGGGCCT [GA]G^G[ACGT]CC[CT]
317 AGGCCCT
DsaI 231 CCACGG C^C[GA][CT]GG
383 CCGTGG
EaeI 191 TGGCCA [CT]^GGCC[GA]
323 TGGCCA
EcoO109I 101 GGGGCCT [GA]G^G[ACGT]CC[CT]
317 AGGCCCT
MboI 114 GATC ^GATC
207 GATC
MluNI 191 TGGCCA TGG^CCA
323 TGGCCA
MscI 191 TGGCCA TGG^CCA
323 TGGCCA
MslI 330 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
361 CACTACCATG
MspA1I 11 CAGCGG C[AC]G^C[GT]G
281 CCGCTG
MwoI 4 GCCCCCGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
66 GCCCGACTTGC
NdeII 114 GATC ^GATC
207 GATC
NlaIII 367 CATG CATG^
424 CATG
Sau3AI 114 GATC ^GATC
207 GATC
TspRI 39 CAGTG CA[GC]TG[ACGT][ACGT]^
274 CACTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstNI Cfr13I EcoRII Fnu4HI
HaeIII HphI ItaI MboII MnlI
MvaI NlaIV Sau96I ScrFI TseI