##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MluI        MluNI       MnlI        MroI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI          427  GTAC                 GT^AC
AluI           45  AGCT                 AG^CT
AseI          157  ATTAAT               AT^TAAT
AsnI          157  ATTAAT               AT^TAAT
BbvI          307  GCTGC                GCAGC(8/12)
BclI          374  TGATCA               T^GATCA
BglII         112  AGATCT               A^GATCT
BsaJI         336  CCTTGG               C^C[ACGT][ACGT]GG
BsmI          265  GCATTC               GAATGC(1/-1)
Bsp1407I      426  TGTACA               T^GTACA
BsrDI         290  GCAATG               GCAATG(2/0)
BsrGI         426  TGTACA               T^GTACA
BstF5I        285  GGATG                GGATG(2/0)
BstYI         112  AGATCT               [GA]^GATC[CT]
EcoRI          34  GAATTC               G^AATTC
EcoT14I       336  CCTTGG               C^C[AT][AT]GG
FbaI          374  TGATCA               T^GATCA
Fnu4HI        307  GCTGC                GC^[ACGT]GC
FokI          285  GGATG                GGATG(9/13)
HincII        348  GTTAAC               GT[CT]^[GA]AC
HindII        348  GTTAAC               GT[CT]^[GA]AC
HindIII        44  AAGCTT               A^AGCTT
HinfI         126  GAATC                G^A[ACGT]TC
HpaI          348  GTTAAC               GTT^AAC
ItaI          307  GCTGC                GC^[ACGT]GC
MaeIII        144  GTTAC                ^GT[ACGT]AC
MfeI          188  CAATTG               C^AATTG
MflI          112  AGATCT               [GA]^GATC[CT]
MunI          188  CAATTG               C^AATTG
PshBI         157  ATTAAT               AT^TAAT
PstI          308  CTGCAG               CTGCA^G
RsaI          427  GTAC                 GT^AC
SapI           19  GAAGAGC              GCTCTTC(1/4)
SfaNI         169  GATGC                GCATC(5/9)
SfcI          308  CTGCAG               C^T[GA][CT]AG
SspBI         426  TGTACA               T^GTACA
StyI          336  CCTTGG               C^C[AT][AT]GG
TfiI          126  GAATC                G^A[AT]TC
TseI          307  GCTGC                G^C[AT]GC
TspRI         243  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         112  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI           34  GAATTC               [GA]^AATT[CT]
              202  GAATTT              
ApoI           34  GAATTC               [GA]^AATT[CT]
              202  GAATTT              
DpnII         113  GATC                 ^GATC
              375  GATC                
EarI           19  GAAGAG               CTCTTC(1/4)
              418  GAAGAG              
HphI           70  GGTGA                GGTGA(8/7)
              397  GGTGA               
Ksp632I        19  GAAGAG               CTCTTC(1/4)
              418  GAAGAG              
MboI          113  GATC                 ^GATC
              375  GATC                
MseI          158  TTAA                 T^TAA
              349  TTAA                
NdeII         113  GATC                 ^GATC
              375  GATC                
NlaIII        153  CATG                 CATG^
              322  CATG                
Sau3AI        113  GATC                 ^GATC
              375  GATC                
Tru9I         158  TTAA                 T^TAA
              349  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       Tsp509I