Restriction map for
pF1KB5476
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboII MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 140 GCGG CCGC(-3/-1)
AcsI 215 GAATTT [GA]^AATT[CT]
AfaI 399 GTAC GT^AC
AocI 193 CCTAAGG CC^T[ACGT]AGG
ApoI 215 GAATTT [GA]^AATT[CT]
AseI 16 ATTAAT AT^TAAT
AsnI 16 ATTAAT AT^TAAT
AvaI 413 CTCGGG C^[CT]CG[GA]G
BbvI 245 GCAGC GCAGC(8/12)
BcgI 248 GCATTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 355 CTAG C^TAG
BpmI 50 CTCCAG CTGGAG(16/14)
BsiYI 130 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 130 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 254 GTCTC GTCTC(1/5)
BsmFI 114 GGGAC GGGAC(10/14)
BsoBI 413 CTCGGG C^[CT]CG[GA]G
BspMI 67 GCAGGT ACCTGC(4/8)
BsrI 73 CCAGT ACTGG(1/-1)
BstXI 104 CCACAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 374 GGATCT [GA]^GATC[CT]
Bsu36I 193 CCTAAGG CC^T[ACGT]AGG
CfoI 4 GCGC GCG^C
DraII 114 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 193 CCTAAGG CC^T[ACGT]AGG
EcoO109I 114 GGGACCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 245 GCAGC GC^[ACGT]GC
HaeII 3 GGCGCT [GA]GCGC^[CT]
HaeIII 39 GGCC GG^CC
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HinfI 423 GACTC G^A[ACGT]TC
ItaI 245 GCAGC GC^[ACGT]GC
MaeI 355 CTAG C^TAG
MaeIII 44 GTGAC ^GT[ACGT]AC
MflI 374 GGATCT [GA]^GATC[CT]
MnlI 49 CCTC CCTC(7/6)
NlaIII 438 CATG CATG^
NlaIV 114 GGGACC GG[ACGT]^[ACGT]CC
PleI 423 GACTC GAGTC(4/5)
PpuMI 114 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 16 ATTAAT AT^TAAT
PstI 65 CTGCAG CTGCA^G
RsaI 399 GTAC GT^AC
SspI 229 AATATT AAT^ATT
TaqI 257 TCGA T^CGA
TseI 245 GCAGC G^C[AT]GC
Tsp45I 44 GTGAC ^GT[GC]AC
TthHB8I 257 TCGA T^CGA
XhoII 374 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
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AlwI 334 GATCC GGATC(4/5)
374 GGATC
AvaII 70 GGTCC G^G[AT]CC
115 GGACC
BstF5I 223 CATCC GGATG(2/0)
81 GGATG
Cac8I 99 GCAAGC GC[ACGT]^[ACGT]GC
282 GCCTGC
FokI 223 CATCC GGATG(9/13)
81 GGATG
MslI 105 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
323 CACTGCTATG
SfcI 65 CTGCAG C^T[GA][CT]AG
170 CTACAG
TspRI 274 CAGTG CA[GC]TG[ACGT][ACGT]^
323 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
Cfr13I DdeI DpnII MboI MseI
NdeII Sau3AI Sau96I Tru9I Tsp509I