##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbsI        BbvCI       
BcgI        BcnI        BfaI        BfrI        BglII       
BlnI        BmyI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FokI        FseI        FspI        
HapII       HincII      HindII      HindIII     HpaI        
HpaII       HphI        KpnI        KspI        MaeI        
MaeII       MamI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvnI        NaeI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI          522  AAATTT               [GA]^AATT[CT]
AcyI          163  GGCGCC               G[GA]^CG[CT]C
ApoI          522  AAATTT               [GA]^AATT[CT]
BanI          163  GGCGCC               G^G[CT][GA]CC
BbeI          163  GGCGCC               GGCGC^C
BciVI         615  GGATAC               GTATCC(6/5)
BclI          653  TGATCA               T^GATCA
BglI           79  GCCATGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         163  GGCGCC               G[GA]^CG[CT]C
BsaJI         702  CCAGGG               C^C[ACGT][ACGT]GG
BsmFI         671  GGGAC                GGGAC(10/14)
BsrBI         171  CCGCTC               CCGCTC(-3/-3)
BsrDI         287  GCAATG               GCAATG(2/0)
CfoI          164  GCGC                 GCG^C
DpnII         654  GATC                 ^GATC
DraIII        681  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
FauI          167  CCCGC                CCCGC(4/6)
FbaI          653  TGATCA               T^GATCA
HaeII         163  GGCGCC               [GA]GCGC^[CT]
HaeIII        567  GGCC                 GG^CC
HgaI          718  GACGC                GACGC(5/10)
HhaI          164  GCGC                 GCG^C
Hin1I         163  GGCGCC               G[GA]^CG[CT]C
HinP1I        164  GCGC                 G^CGC
KasI          163  GGCGCC               G^GCGCC
MboI          654  GATC                 ^GATC
MfeI          602  CAATTG               C^AATTG
MunI          602  CAATTG               C^AATTG
NarI          163  GGCGCC               GG^CGCC
NdeII         654  GATC                 ^GATC
NlaIV         163  GGCGCC               GG[ACGT]^[ACGT]CC
PleI          631  GAGTC                GAGTC(4/5)
SapI          173  GCTCTTC              GCTCTTC(1/4)
Sau3AI        654  GATC                 ^GATC
SfaNI         403  GCATC                GCATC(5/9)
SfoI          163  GGCGCC               GGC^GCC
StuI          566  AGGCCT               AGG^CCT
TaqI          433  TCGA                 T^CGA
TfiI          546  GATTC                G^A[AT]TC
Tsp45I         91  GTCAC                ^GT[GC]AC
TspRI         426  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       433  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          168  CCGC                 CCGC(-3/-1)
              171  CCGC                
AfaI           25  GTAC                 GT^AC
              249  GTAC                
AluI           20  AGCT                 AG^CT
              302  AGCT                
BlpI           43  GCTGAGC              GC^T[ACGT]AGC
               52  GCTGAGC             
Bpu1102I       43  GCTGAGC              GC^T[ACGT]AGC
               52  GCTGAGC             
BsmBI         707  GAGACG               CGTCTC(1/5)
              716  GAGACG              
CelII          43  GCTGAGC              GC^T[ACGT]AGC
               52  GCTGAGC             
HinfI         546  GATTC                G^A[ACGT]TC
              631  GAGTC               
MaeIII         91  GTCAC                ^GT[ACGT]AC
              663  GTTAC               
PstI           74  CTGCAG               CTGCA^G
              274  CTGCAG              
RsaI           25  GTAC                 GT^AC
              249  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

BbvI        BpmI        BsmAI       BssKI       BstNI       
Cac8I       DdeI        EarI        EcoRII      Fnu4HI      
ItaI        Ksp632I     MboII       MnlI        MvaI        
MwoI        NlaIII      ScrFI       SfcI        TseI        
Tsp509I