Restriction map for
pF1KB5370
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 346 GTCTAC GT^[AC][GT]AC
AcsI 370 AAATTT [GA]^AATT[CT]
Alw44I 361 GTGCAC G^TGCAC
AlwNI 397 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 361 GTGCAC G^TGCAC
ApoI 370 AAATTT [GA]^AATT[CT]
AspHI 361 GTGCAC G[AT]GC[AT]^C
AvaI 518 CCCGAG C^[CT]CG[GA]G
BamHI 5 GGATCC G^GATCC
BfaI 154 CTAG C^TAG
BpmI 803 CTCCAG CTGGAG(16/14)
BsaBI 310 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 850 GTGCAG GTGCAG(16/14)
BsiHKAI 361 GTGCAC G[AT]GC[AT]^C
BsmAI 393 GTCTC GTCTC(1/5)
BsoBI 518 CCCGAG C^[CT]CG[GA]G
BsrBI 800 CCGCTC CCGCTC(-3/-3)
BstXI 739 CCAAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 94 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 376 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 94 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 655 GATATC GAT^ATC
MaeI 154 CTAG C^TAG
MamI 310 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 564 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 780 CATG CATG^
PflMI 216 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 94 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 685 CTGCAG CTGCA^G
SfaNI 900 GATGC GCATC(5/9)
Van91I 216 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 106 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
AvaII 95 GGTCC G^G[AT]CC
123 GGACC
BanII 15 GAGCCC G[GA]GC[CT]^C
75 GGGCTC
BcnI 585 CCGGG CC^[GC]GG
786 CCGGG
BspMI 140 ACCTGC ACCTGC(4/8)
794 ACCTGC
BsrFI 101 GCCGGC [GA]^CCGG[CT]
547 GCCGGC
Bsu36I 106 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
Cfr10I 101 GCCGGC [GA]^CCGG[CT]
547 GCCGGC
Eco81I 106 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
HinfI 560 GAATC G^A[ACGT]TC
905 GATTC
MseI 482 TTAA T^TAA
710 TTAA
MspA1I 577 CAGCTG C[AC]G^C[GT]G
853 CAGCTG
NaeI 101 GCCGGC GCC^GGC
547 GCCGGC
NciI 585 CCGGG CC^[GC]GG
786 CCGGG
NgoMIV 101 GCCGGC G^CCGGC
547 GCCGGC
PvuII 577 CAGCTG CAG^CTG
853 CAGCTG
TfiI 560 GAATC G^A[AT]TC
905 GATTC
Tru9I 482 TTAA T^TAA
710 TTAA
Tsp509I 264 AATT ^AATT
371 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI XhoII