Restriction map for
pF1KB5297
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 274 GTAGAC GT^[AC][GT]AC
AlwNI 125 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 715 CCTGAGG CC^T[ACGT]AGG
BalI 492 TGGCCA TGG^CCA
BamHI 812 GGATCC G^GATCC
BanI 250 GGTGCC G^G[CT][GA]CC
BfaI 55 CTAG C^TAG
BseRI 87 GAGGAG GAGGAG(10/8)
BsrDI 472 GCAATG GCAATG(2/0)
BsrFI 103 ACCGGC [GA]^CCGG[CT]
BssKI 831 CCTGG ^CC[ACGT]GG
BstF5I 18 CATCC GGATG(2/0)
BstNI 831 CCTGG CC^[AT]GG
Bsu36I 715 CCTGAGG CC^T[ACGT]AGG
Cac8I 182 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 895 GCGC GCG^C
Cfr10I 103 ACCGGC [GA]^CCGG[CT]
DraI 840 TTTAAA TTT^AAA
DraII 699 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 489 CCATGG C^C[GA][CT]GG
EaeI 492 TGGCCA [CT]^GGCC[GA]
Eco81I 715 CCTGAGG CC^T[ACGT]AGG
EcoO109I 699 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 831 CCTGG ^CC[AT]GG
EcoT22I 739 ATGCAT ATGCA^T
FokI 18 CATCC GGATG(9/13)
HaeIII 493 GGCC GG^CC
HapII 104 CCGG C^CGG
HhaI 895 GCGC GCG^C
HinP1I 895 GCGC G^CGC
HindIII 70 AAGCTT A^AGCTT
HpaII 104 CCGG C^CGG
MaeI 55 CTAG C^TAG
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
MslI 641 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 104 CCGG C^CGG
MvaI 831 CCTGG CC^[AT]GG
MwoI 511 GCTTCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 489 CCATGG C^CATGG
NsiI 739 ATGCAT ATGCA^T
PpuMI 699 GGGACCC [GA]G^G[AT]CC[CT]
PstI 279 CTGCAG CTGCA^G
ScrFI 831 CCTGG CC^[ACGT]GG
SgrAI 102 CACCGGCG C[GA]^CCGG[CT]G
TfiI 747 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 4 AAATTT [GA]^AATT[CT]
163 GAATTT
AfaI 433 GTAC GT^AC
689 GTAC
AflIII 525 ACATGT A^C[GA][CT]GT
640 ACATGT
ApoI 4 AAATTT [GA]^AATT[CT]
163 GAATTT
AvaII 534 GGTCC G^G[AT]CC
700 GGACC
BpmI 217 CTGGAG CTGGAG(16/14)
626 CTGGAG
BsaJI 254 CCAAGG C^C[ACGT][ACGT]GG
489 CCATGG
BsmAI 147 GAGAC GTCTC(1/5)
529 GTCTC
BspLU11I 525 ACATGT A^CATGT
640 ACATGT
BstYI 399 AGATCC [GA]^GATC[CT]
812 GGATCC
Cfr13I 534 GGTCC G^G[ACGT]CC
700 GGACC
EcoT14I 254 CCAAGG C^C[AT][AT]GG
489 CCATGG
HphI 318 GGTGA GGTGA(8/7)
101 TCACC
MflI 399 AGATCC [GA]^GATC[CT]
812 GGATCC
MspA1I 227 CAGCTG C[AC]G^C[GT]G
518 CAGCTG
NspI 525 ACATGT [GA]CATG^[CT]
640 ACATGT
PleI 58 GAGTC GAGTC(4/5)
629 GAGTC
PvuII 227 CAGCTG CAG^CTG
518 CAGCTG
RsaI 433 GTAC GT^AC
689 GTAC
Sau96I 534 GGTCC G^G[ACGT]CC
700 GGACC
SfaNI 738 GATGC GCATC(5/9)
868 GATGC
SfcI 279 CTGCAG C^T[GA][CT]AG
785 CTATAG
StyI 254 CCAAGG C^C[AT][AT]GG
489 CCATGG
Tsp45I 100 GTCAC ^GT[GC]AC
502 GTCAC
XhoII 399 AGATCC [GA]^GATC[CT]
812 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AlwI BbvI BsmFI BsrI
DdeI DpnII Fnu4HI HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV Sau3AI Tru9I
TseI Tsp509I TspRI