Restriction map for
pF1KE9696
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# NO CUT ENZYMES ( 53 enzymes) #
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AatII AclI Alw44I ApaLI AscI
AseI AsnI AviII AvrII BamHI
BciVI BglI BlnI BsaI BsiWI
BsmI Bsp1407I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I EagI EclXI
Eco52I EcoT22I FspI KspI MfeI
MunI NheI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
SacII SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 4886 GGTACC G^GTACC
AflII 3061 CTTAAG C^TTAAG
AgeI 3533 ACCGGT A^CCGGT
AhdI 4060 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4565 AGCGCT AGC^GCT
Asp718I 4886 GGTACC G^GTACC
AspEI 4060 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 5827 GGCGCC GGCGC^C
BbrPI 1971 CACGTG CAC^GTG
BcgI 1483 CGATGCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3061 CTTAAG C^TTAAG
BglII 5201 AGATCT A^GATCT
BsaAI 1971 CACGTG [CT]AC^GT[GA]
BsiEI 3345 CGATCG CG[GA][CT]^CG
BspLU11I 2198 ACATGT A^CATGT
BstAPI 2856 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5026 GGTGACC G^GT[ACGT]ACC
BstPI 5026 GGTGACC G^GT[ACGT]ACC
BstXI 1143 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 291 CGGACCG CG^G[AT]CCG
DraI 5314 TTTAAA TTT^AAA
DrdI 3543 GACTCGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4060 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4565 AGCGCT AGC^GCT
EcoO65I 5026 GGTGACC G^GT[ACGT]ACC
EcoRV 745 GATATC GAT^ATC
HpaI 4027 GTTAAC GTT^AAC
KasI 5827 GGCGCC G^GCGCC
KpnI 4886 GGTACC GGTAC^C
MluI 1403 ACGCGT A^CGCGT
NarI 5827 GGCGCC GG^CGCC
NdeI 1958 CATATG CA^TATG
PinAI 3533 ACCGGT A^CCGGT
PmaCI 1971 CACGTG CAC^GTG
PmlI 1971 CACGTG CAC^GTG
PshAI 928 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3345 CGATCG CGAT^CG
RsrII 291 CGGACCG CG^G[AT]CCG
SalI 4920 GTCGAC G^TCGAC
SbfI 5848 CCTGCAGG CCTGCA^GG
SfoI 5827 GGCGCC GGC^GCC
Sse8387I 5848 CCTGCAGG CCTGCA^GG
XbaI 4836 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 4130 GTAGAC GT^[AC][GT]AC
4920 GTCGAC
AccIII 709 TCCGGA T^CCGGA
4151 TCCGGA
AcyI 4923 GACGCC G[GA]^CG[CT]C
5827 GGCGCC
AflIII 1403 ACGCGT A^C[GA][CT]GT
2198 ACATGT
BsaBI 555 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1579 GATGGAAATC
BsaHI 4923 GACGCC G[GA]^CG[CT]C
5827 GGCGCC
BseAI 709 TCCGGA T^CCGGA
4151 TCCGGA
BsmBI 4598 CGTCTC CGTCTC(1/5)
1111 GAGACG
BspDI 3304 ATCGAT AT^CGAT
3343 ATCGAT
BspEI 709 TCCGGA T^CCGGA
4151 TCCGGA
BspHI 503 TCATGA T^CATGA
3050 TCATGA
BsrBI 2389 GAGCGG CCGCTC(-3/-3)
4810 GAGCGG
BssSI 3014 CACGAG CACGAG(-5/-1)
1181 CTCGTG
ClaI 3304 ATCGAT AT^CGAT
3343 ATCGAT
EcoRI 2476 GAATTC G^AATTC
3009 GAATTC
FseI 994 GGCCGGCC GGCCGG^CC
1036 GGCCGGCC
Hin1I 4923 GACGCC G[GA]^CG[CT]C
5827 GGCGCC
HindIII 847 AAGCTT A^AGCTT
2516 AAGCTT
MamI 555 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1579 GATGGAAATC
MroI 709 TCCGGA T^CCGGA
4151 TCCGGA
NcoI 5250 CCATGG C^CATGG
5465 CCATGG
PaeR7I 921 CTCGAG C^TCGAG
5051 CTCGAG
PstI 1076 CTGCAG CTGCA^G
5849 CTGCAG
RcaI 503 TCATGA T^CATGA
3050 TCATGA
SexAI 3029 ACCAGGT A^CC[AT]GGT
3605 ACCTGGT
XhoI 921 CTCGAG C^TCGAG
5051 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI