Restriction map for
pF1KE2267
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoRV FbaI FseI FspI HaeII
HindIII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1708 GTAGAC GT^[AC][GT]AC
Asp700I 1603 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 727 CACGTG CAC^GTG
BbvCI 2553 CCTCAGC CCTCAGC(-5/-2)
BglII 2249 AGATCT A^GATCT
BsaAI 727 CACGTG [CT]AC^GT[GA]
BsaI 2004 GGTCTC GGTCTC(1/5)
BsmBI 535 GAGACG CGTCTC(1/5)
BspLU11I 2761 ACATGT A^CATGT
BspMI 59 GCAGGT ACCTGC(4/8)
BstEII 580 GGTGACC G^GT[ACGT]ACC
BstPI 580 GGTGACC G^GT[ACGT]ACC
CfoI 98 GCGC GCG^C
DraIII 964 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 70 CGGCCG C^GGCCG
EclXI 70 CGGCCG C^GGCCG
Eco52I 70 CGGCCG C^GGCCG
EcoNI 1188 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 580 GGTGACC G^GT[ACGT]ACC
EcoRI 1435 GAATTC G^AATTC
EcoT22I 1577 ATGCAT ATGCA^T
HhaI 98 GCGC GCG^C
HinP1I 98 GCGC G^CGC
HincII 1513 GTTGAC GT[CT]^[GA]AC
HindII 1513 GTTGAC GT[CT]^[GA]AC
NcoI 1102 CCATGG C^CATGG
NotI 69 GCGGCCGC GC^GGCCGC
NsiI 1577 ATGCAT ATGCA^T
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
PvuII 1068 CAGCTG CAG^CTG
SapI 1566 GAAGAGC GCTCTTC(1/4)
ScaI 182 AGTACT AGT^ACT
SmlI 917 CTCAAG C^T[CT][GA]AG
SphI 37 GCATGC GCATG^C
TaqI 1812 TCGA T^CGA
TthHB8I 1812 TCGA T^CGA
XmnI 1603 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 702 GACGCC G[GA]^CG[CT]C
2207 GACGCC
AlwNI 1988 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
2750 CAGGTGCTG
ApaI 1184 GGGCCC GGGCC^C
1667 GGGCCC
BamHI 115 GGATCC G^GATCC
1728 GGATCC
BanI 2335 GGCACC G^G[CT][GA]CC
2434 GGCACC
BsaHI 702 GACGCC G[GA]^CG[CT]C
2207 GACGCC
BseRI 2613 CTCCTC GAGGAG(10/8)
178 GAGGAG
BsiEI 70 CGGCCG CG[GA][CT]^CG
1062 CGACCG
BsmI 682 GAATGC GAATGC(1/-1)
2603 GAATGC
BsrDI 945 CATTGC GCAATG(2/0)
2012 GCAATG
BssSI 1612 CACGAG CACGAG(-5/-1)
1960 CACGAG
FauI 75 GCGGG CCCGC(4/6)
100 GCGGG
HgaI 702 GACGC GACGC(5/10)
2207 GACGC
Hin1I 702 GACGCC G[GA]^CG[CT]C
2207 GACGCC
KspI 24 CCGCGG CCGC^GG
73 CCGCGG
SacII 24 CCGCGG CCGC^GG
73 CCGCGG
SspI 1629 AATATT AAT^ATT
2455 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmaCI XmaI