Restriction map for
pF1KB7354
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstEII BstPI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 2865 CTTAAG C^TTAAG
AspHI 362 GAGCTC G[AT]GC[AT]^C
BclI 671 TGATCA T^GATCA
BcnI 3369 CCCGG CC^[GC]GG
BfrI 2865 CTTAAG C^TTAAG
BsgI 1518 CTGCAC GTGCAG(16/14)
BsiHKAI 362 GAGCTC G[AT]GC[AT]^C
BspHI 1137 TCATGA T^CATGA
BspMI 3334 GCAGGT ACCTGC(4/8)
BsrDI 1502 GCAATG GCAATG(2/0)
BstAPI 3382 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2979 CCAGATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 6 GCGC GCG^C
DraI 1465 TTTAAA TTT^AAA
DraIII 147 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1841 GAATTC G^AATTC
EcoRV 3259 GATATC GAT^ATC
EcoT14I 958 CCTTGG C^C[AT][AT]GG
EcoT22I 1574 ATGCAT ATGCA^T
FauI 1968 GCGGG CCCGC(4/6)
FbaI 671 TGATCA T^GATCA
HaeII 5 GGCGCT [GA]GCGC^[CT]
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
MfeI 2230 CAATTG C^AATTG
MunI 2230 CAATTG C^AATTG
NciI 3369 CCCGG CC^[GC]GG
NsiI 1574 ATGCAT ATGCA^T
PflMI 681 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1137 TCATGA T^CATGA
SacI 362 GAGCTC GAGCT^C
SapI 1186 GAAGAGC GCTCTTC(1/4)
SnaBI 67 TACGTA TAC^GTA
SspI 2749 AATATT AAT^ATT
StyI 958 CCTTGG C^C[AT][AT]GG
Van91I 681 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AseI 1270 ATTAAT AT^TAAT
1294 ATTAAT
AsnI 1270 ATTAAT AT^TAAT
1294 ATTAAT
Asp700I 454 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2354 GAAATTGTTC
BanI 1551 GGCACC G^G[CT][GA]CC
1752 GGCACC
BanII 362 GAGCTC G[GA]GC[CT]^C
3162 GGGCTC
BmyI 362 GAGCTC G[AGT]GC[ACT]^C
3162 GGGCTC
BsaAI 67 TACGTA [CT]AC^GT[GA]
1207 TACGTG
BsmI 625 GAATGC GAATGC(1/-1)
3404 GAATGC
Bsp1286I 362 GAGCTC G[AGT]GC[ACT]^C
3162 GGGCTC
Bsp1407I 1491 TGTACA T^GTACA
2634 TGTACA
BspLU11I 555 ACATGT A^CATGT
3244 ACATGT
BsrGI 1491 TGTACA T^GTACA
2634 TGTACA
Bst1107I 778 GTATAC GTA^TAC
924 GTATAC
BstBI 380 TTCGAA TT^CGAA
437 TTCGAA
BstZ17I 778 GTATAC GTA^TAC
924 GTATAC
NspV 380 TTCGAA TT^CGAA
437 TTCGAA
PshBI 1270 ATTAAT AT^TAAT
1294 ATTAAT
ScaI 1568 AGTACT AGT^ACT
3301 AGTACT
SfuI 380 TTCGAA TT^CGAA
437 TTCGAA
SmlI 1160 CTCAAG C^T[CT][GA]AG
2865 CTTAAG
SspBI 1491 TGTACA T^GTACA
2634 TGTACA
XcmI 1287 CCAAGAAATTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3224 CCATAATCCTCATGG
XmnI 454 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2354 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaII
BalI BamHI BbsI BbvI BfaI
BpuAI BsaBI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII