Restriction map for
pF1KB7351
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccIII AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AvrII BalI BbeI
BbrPI BbvCI BciVI BfrI BglII
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstPI BstXI Cfr10I ClaI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
HpaI KasI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 3346 GACGTC GACGT^C
Acc65I 2766 GGTACC G^GTACC
AclI 3674 AACGTT AA^CGTT
AseI 3550 ATTAAT AT^TAAT
AsnI 3550 ATTAAT AT^TAAT
Asp718I 2766 GGTACC G^GTACC
AvaI 2249 CTCGGG C^[CT]CG[GA]G
AviII 1541 TGCGCA TGC^GCA
BamHI 3096 GGATCC G^GATCC
BcgI 1911 GCAGCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1824 TGATCA T^GATCA
BglI 148 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1294 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1294 GCTGAGC GC^T[ACGT]AGC
BsaBI 243 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3451 GGTCTC GGTCTC(1/5)
BsmBI 1314 GAGACG CGTCTC(1/5)
BsoBI 2249 CTCGGG C^[CT]CG[GA]G
BssSI 3068 CACGAG CACGAG(-5/-1)
Bst1107I 1041 GTATAC GTA^TAC
BstZ17I 1041 GTATAC GTA^TAC
CelII 1294 GCTGAGC GC^T[ACGT]AGC
CpoI 38 CGGTCCG CG^G[AT]CCG
DraIII 61 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2936 CCATGG C^C[GA][CT]GG
EcoNI 1204 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2426 GAATTC G^AATTC
EcoT22I 1655 ATGCAT ATGCA^T
FbaI 1824 TGATCA T^GATCA
FspI 1541 TGCGCA TGC^GCA
HaeII 1558 AGCGCC [GA]GCGC^[CT]
HindIII 223 AAGCTT A^AGCTT
KpnI 2766 GGTACC GGTAC^C
MamI 243 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2936 CCATGG C^CATGG
NsiI 1655 ATGCAT ATGCA^T
PpuMI 702 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3550 ATTAAT AT^TAAT
Psp1406I 3674 AACGTT AA^CGTT
PstI 1736 CTGCAG CTGCA^G
PvuII 3691 CAGCTG CAG^CTG
RsrII 38 CGGTCCG CG^G[AT]CCG
SacI 2235 GAGCTC GAGCT^C
SalI 3753 GTCGAC G^TCGAC
ScaI 607 AGTACT AGT^ACT
SpeI 3259 ACTAGT A^CTAGT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 43 CGCG CG^CG
80 CGCG
AcyI 2864 GGCGTC G[GA]^CG[CT]C
3346 GACGTC
AlwNI 1346 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3745 CAGGGCCTG
AocI 302 CCTTAGG CC^T[ACGT]AGG
2995 CCTAAGG
BcnI 74 CCCGG CC^[GC]GG
2763 CCCGG
BsaHI 2864 GGCGTC G[GA]^CG[CT]C
3346 GACGTC
BsmFI 1492 GTCCC GGGAC(10/14)
2991 GTCCC
BsrBI 10 CCGCTC CCGCTC(-3/-3)
87 CCGCTC
BstAPI 3135 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3237 GCAAAGAATGC
BstUI 43 CGCG CG^CG
80 CGCG
Bsu36I 302 CCTTAGG CC^T[ACGT]AGG
2995 CCTAAGG
DraII 702 GGGTCCT [GA]G^G[ACGT]CC[CT]
3746 AGGGCCT
EaeI 20 TGGCCG [CT]^GGCC[GA]
155 TGGCCG
Eco81I 302 CCTTAGG CC^T[ACGT]AGG
2995 CCTAAGG
EcoO109I 702 GGGTCCT [GA]G^G[ACGT]CC[CT]
3746 AGGGCCT
EcoT14I 1563 CCTTGG C^C[AT][AT]GG
2936 CCATGG
FauI 9 CCCGC CCCGC(4/6)
86 CCCGC
HapII 75 CCGG C^CGG
2764 CCGG
Hin1I 2864 GGCGTC G[GA]^CG[CT]C
3346 GACGTC
HpaII 75 CCGG C^CGG
2764 CCGG
MslI 195 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2587 CACTACTGTG
MspA1I 23 CCGCTG C[AC]G^C[GT]G
3691 CAGCTG
MspI 75 CCGG C^CGG
2764 CCGG
MvnI 43 CGCG CG^CG
80 CGCG
NciI 74 CCCGG CC^[GC]GG
2763 CCCGG
PleI 1550 GACTC GAGTC(4/5)
621 GAGTC
StyI 1563 CCTTGG C^C[AT][AT]GG
2936 CCATGG
XbaI 54 TCTAGA T^CTAGA
2154 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HgaI
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeI NdeII
NlaIII NlaIV RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI