Restriction map for
pF1KE9260
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BcgI BclI BfrI BlnI BsaAI
BsiEI BsiWI BspDI BsrBI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SgrAI SmaI
SnaBI SpeI SphI SwaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4086 GTCTAC GT^[AC][GT]AC
AccIII 1781 TCCGGA T^CCGGA
BamHI 2430 GGATCC G^GATCC
BbeI 4679 GGCGCC GGCGC^C
BbvCI 4027 CCTCAGC CCTCAGC(-5/-2)
BciVI 707 GGATAC GTATCC(6/5)
BlpI 4224 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4224 GCTCAGC GC^T[ACGT]AGC
BsaI 2282 GAGACC GGTCTC(1/5)
BsaWI 1781 TCCGGA [AT]^CCGG[AT]
BseAI 1781 TCCGGA T^CCGGA
BsgI 1088 CTGCAC GTGCAG(16/14)
BsmI 1785 GAATGC GAATGC(1/-1)
Bsp1407I 2206 TGTACA T^GTACA
BspEI 1781 TCCGGA T^CCGGA
BsrFI 2664 ACCGGC [GA]^CCGG[CT]
BsrGI 2206 TGTACA T^GTACA
BssSI 3521 CTCGTG CACGAG(-5/-1)
CelII 4224 GCTCAGC GC^T[ACGT]AGC
CfoI 4680 GCGC GCG^C
Cfr10I 2664 ACCGGC [GA]^CCGG[CT]
DraI 2857 TTTAAA TTT^AAA
DraIII 4331 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4312 CCTATGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1474 GATATC GAT^ATC
FauI 47 GCGGG CCCGC(4/6)
HaeII 4679 GGCGCC [GA]GCGC^[CT]
HhaI 4680 GCGC GCG^C
HinP1I 4680 GCGC G^CGC
HincII 78 GTCAAC GT[CT]^[GA]AC
HindII 78 GTCAAC GT[CT]^[GA]AC
KasI 4679 GGCGCC G^GCGCC
MfeI 3881 CAATTG C^AATTG
MroI 1781 TCCGGA T^CCGGA
MunI 3881 CAATTG C^AATTG
NarI 4679 GGCGCC GG^CGCC
PaeR7I 1598 CTCGAG C^TCGAG
PpuMI 933 AGGTCCT [GA]G^G[AT]CC[CT]
SbfI 3728 CCTGCAGG CCTGCA^GG
SfoI 4679 GGCGCC GGC^GCC
Sse8387I 3728 CCTGCAGG CCTGCA^GG
SspBI 2206 TGTACA T^GTACA
SspI 834 AATATT AAT^ATT
XhoI 1598 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 602 GACGTC GACGT^C
4588 GACGTC
AccII 3286 CGCG CG^CG
4585 CGCG
AflIII 3464 ACATGT A^C[GA][CT]GT
3679 ACATGT
AhdI 4424 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4583 GACGCGACGTC
ApaI 1875 GGGCCC GGGCC^C
4649 GGGCCC
AspEI 4424 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4583 GACGCGACGTC
AspHI 208 GAGCTC G[AT]GC[AT]^C
4222 GAGCTC
AspI 2179 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTGTGTC
AvaI 1598 CTCGAG C^[CT]CG[GA]G
3260 CTCGGG
BcnI 4405 CCCGG CC^[GC]GG
4701 CCGGG
BglI 2816 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4374 GCCTCCAGGGC
BsaBI 282 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
751 GATATAGATC
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
4222 GAGCTC
BsmBI 604 CGTCTC CGTCTC(1/5)
1469 GAGACG
BsoBI 1598 CTCGAG C^[CT]CG[GA]G
3260 CTCGGG
BspLU11I 3464 ACATGT A^CATGT
3679 ACATGT
BspMI 2901 ACCTGC ACCTGC(4/8)
3667 ACCTGC
BstAPI 766 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1431 GCAAGTTATGC
BstUI 3286 CGCG CG^CG
4585 CGCG
BstXI 2108 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4049 CCAGTGAGCTGG
DrdI 926 GACTGACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2302 GACTCTCATGTC
Eam1105I 4424 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4583 GACGCGACGTC
HgaI 4583 GACGC GACGC(5/10)
3516 GCGTC
MamI 282 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
751 GATATAGATC
MvnI 3286 CGCG CG^CG
4585 CGCG
NciI 4405 CCCGG CC^[GC]GG
4701 CCGGG
NdeI 2527 CATATG CA^TATG
4346 CATATG
PflFI 2179 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTGTGTC
PflMI 3504 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4299 CCAAGGAGTGG
PvuII 2960 CAGCTG CAG^CTG
4418 CAGCTG
SacI 208 GAGCTC GAGCT^C
4222 GAGCTC
SapI 17 GAAGAGC GCTCTTC(1/4)
210 GCTCTTC
SexAI 3426 ACCAGGT A^CC[AT]GGT
4332 ACCAGGT
StuI 2814 AGGCCT AGG^CCT
4502 AGGCCT
Tth111I 2179 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTGTGTC
Van91I 3504 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4299 CCAAGGAGTGG
XbaI 2065 TCTAGA T^CTAGA
3036 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BalI BanI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstBI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI NspV PleI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI