Restriction map for
pF1KB7295
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BciVI BclI BfaI
BfrI BlnI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI DraI Eco47III Eco81I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MaeI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 946 GTCTAC GT^[AC][GT]AC
AcsI 1998 GAATTC [GA]^AATT[CT]
AflIII 1457 ACATGT A^C[GA][CT]GT
AhdI 880 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1998 GAATTC [GA]^AATT[CT]
AspEI 880 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 864 CACGTG CAC^GTG
BbsI 1690 GTCTTC GAAGAC(2/6)
BbvCI 993 CCTCAGC CCTCAGC(-5/-2)
BpmI 1924 CTGGAG CTGGAG(16/14)
BpuAI 1690 GTCTTC GAAGAC(2/6)
BsaAI 864 CACGTG [CT]AC^GT[GA]
BsaBI 1092 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1957 GAGACG CGTCTC(1/5)
BspLU11I 1457 ACATGT A^CATGT
BsrBI 2233 CCGCTC CCGCTC(-3/-3)
BstAPI 2013 GCACAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2166 GGTCACC G^GT[ACGT]ACC
BstPI 2166 GGTCACC G^GT[ACGT]ACC
BstXI 893 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 2582 CGGACCG CG^G[AT]CCG
DrdI 937 GACTCCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 880 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2166 GGTCACC G^GT[ACGT]ACC
EcoRI 1998 GAATTC G^AATTC
MamI 1092 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 1398 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 864 CACGTG CAC^GTG
PmlI 864 CACGTG CAC^GTG
PvuII 1478 CAGCTG CAG^CTG
RsrII 2582 CGGACCG CG^G[AT]CCG
SacI 1561 GAGCTC GAGCT^C
SexAI 773 ACCTGGT A^CC[AT]GGT
SfiI 208 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2613 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1678 CTCAAG C^T[CT][GA]AG
Tsp509I 1999 AATT ^AATT
Van91I 1398 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 1153 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1399 CAGTCGCTG
AscI 1946 GGCGCGCC GG^CGCGCC
2344 GGCGCGCC
AviII 860 TGCGCA TGC^GCA
968 TGCGCA
BalI 1079 TGGCCA TGG^CCA
1964 TGGCCA
BcgI 797 GCATTGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
863 GCACGTGGTTCG
BglII 1055 AGATCT A^GATCT
1826 AGATCT
BlpI 1335 GCTCAGC GC^T[ACGT]AGC
1896 GCTGAGC
Bpu1102I 1335 GCTCAGC GC^T[ACGT]AGC
1896 GCTGAGC
BsaI 715 GAGACC GGTCTC(1/5)
747 GGTCTC
CelII 1335 GCTCAGC GC^T[ACGT]AGC
1896 GCTGAGC
DraIII 1756 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2014 CACAAGGTG
EarI 1252 CTCTTC CTCTTC(1/4)
2137 CTCTTC
EcoNI 1575 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2010 CCTGCACAAGG
EcoT14I 281 CCAAGG C^C[AT][AT]GG
362 CCAAGG
FspI 860 TGCGCA TGC^GCA
968 TGCGCA
HincII 523 GTCAAC GT[CT]^[GA]AC
1591 GTCAAC
HindII 523 GTCAAC GT[CT]^[GA]AC
1591 GTCAAC
Ksp632I 1252 CTCTTC CTCTTC(1/4)
2137 CTCTTC
MaeII 743 ACGT A^CGT
865 ACGT
MluNI 1079 TGGCCA TGG^CCA
1964 TGGCCA
MscI 1079 TGGCCA TGG^CCA
1964 TGGCCA
NotI 245 GCGGCCGC GC^GGCCGC
2229 GCGGCCGC
NspI 1271 ACATGC [GA]CATG^[CT]
1457 ACATGT
StyI 281 CCAAGG C^C[AT][AT]GG
362 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI