Restriction map for
pF1KB9670
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 268 GTCGAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AlwI 795 GATCC GGATC(4/5)
AvaII 106 GGACC G^G[AT]CC
BanII 3 GAGCCC G[GA]GC[CT]^C
BbvI 404 GCAGC GCAGC(8/12)
BcnI 530 CCCGG CC^[GC]GG
BmyI 3 GAGCCC G[AGT]GC[ACT]^C
BsaI 66 GGTCTC GGTCTC(1/5)
BsiYI 524 CCTTAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 524 CCTTAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 232 GAATGC GAATGC(1/-1)
Bsp1286I 3 GAGCCC G[AGT]GC[ACT]^C
BstAPI 572 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 444 CATCC GGATG(2/0)
BstNI 237 CCAGG CC^[AT]GG
BstUI 13 CGCG CG^CG
BstXI 237 CCAGGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 794 AGATCC [GA]^GATC[CT]
Cfr13I 106 GGACC G^G[ACGT]CC
DpnII 795 GATC ^GATC
EarI 519 CTCTTC CTCTTC(1/4)
EcoRII 237 CCAGG ^CC[AT]GG
Fnu4HI 404 GCAGC GC^[ACGT]GC
FokI 444 CATCC GGATG(9/13)
HaeIII 756 GGCC GG^CC
HapII 531 CCGG C^CGG
HincII 268 GTCGAC GT[CT]^[GA]AC
HindII 268 GTCGAC GT[CT]^[GA]AC
HpaII 531 CCGG C^CGG
ItaI 404 GCAGC GC^[ACGT]GC
Ksp632I 519 CTCTTC CTCTTC(1/4)
MboI 795 GATC ^GATC
MflI 794 AGATCC [GA]^GATC[CT]
MspI 531 CCGG C^CGG
MvaI 237 CCAGG CC^[AT]GG
MvnI 13 CGCG CG^CG
NciI 530 CCCGG CC^[GC]GG
NdeII 795 GATC ^GATC
NlaIV 105 GGGACC GG[ACGT]^[ACGT]CC
PstI 635 CTGCAG CTGCA^G
SalI 268 GTCGAC G^TCGAC
Sau3AI 795 GATC ^GATC
Sau96I 106 GGACC G^G[ACGT]CC
SfaNI 450 GCATC GCATC(5/9)
StuI 755 AGGCCT AGG^CCT
TseI 404 GCAGC G^C[AT]GC
XcmI 372 CCAGATTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 794 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 427 AGCT AG^CT
685 AGCT
Asp700I 333 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
585 GAAGACCTTC
BsmAI 77 GAGAC GTCTC(1/5)
67 GTCTC
BsmFI 25 GGGAC GGGAC(10/14)
105 GGGAC
BspMI 63 GCAGGT ACCTGC(4/8)
347 GCAGGT
BssKI 237 CCAGG ^CC[ACGT]GG
530 CCCGG
FauI 329 GCGGG CCCGC(4/6)
581 GCGGG
MseI 173 TTAA T^TAA
526 TTAA
MslI 88 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
213 CACTGGGGTG
PleI 97 GACTC GAGTC(4/5)
711 GAGTC
ScrFI 237 CCAGG CC^[ACGT]GG
530 CCCGG
SfcI 635 CTGCAG C^T[GA][CT]AG
681 CTACAG
Tru9I 173 TTAA T^TAA
526 TTAA
XmnI 333 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
585 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BbsI BpmI BpuAI BsgI
BsrI HinfI HphI MaeIII MboII
MnlI MwoI NlaIII TaqI TfiI
Tsp45I TspRI TthHB8I