##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        AlwNI       AocI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsiEI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MamI        MfeI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII        668  TCCGGA               T^CCGGA
AcsI          179  GAATTC               [GA]^AATT[CT]
ApoI          179  GAATTC               [GA]^AATT[CT]
AvaI          817  CCCGAG               C^[CT]CG[GA]G
BalI          130  TGGCCA               TGG^CCA
BanI           85  GGTGCC               G^G[CT][GA]CC
BglI          132  GCCAGAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          333  CTCCAG               CTGGAG(16/14)
BsaWI         668  TCCGGA               [AT]^CCGG[AT]
BseAI         668  TCCGGA               T^CCGGA
BsoBI         817  CCCGAG               C^[CT]CG[GA]G
BspEI         668  TCCGGA               T^CCGGA
BspHI         225  TCATGA               T^CATGA
BsrBI         697  GAGCGG               CCGCTC(-3/-3)
BsrI         1323  CCAGT                ACTGG(1/-1)
BstXI         121  CCAGGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRI         179  GAATTC               G^AATTC
EcoT14I       249  CCAAGG               C^C[AT][AT]GG
HgaI         1443  GACGC                GACGC(5/10)
HinfI        1458  GATTC                G^A[ACGT]TC
KspI          142  CCGCGG               CCGC^GG
MaeIII        479  GTGAC                ^GT[ACGT]AC
MluI          725  ACGCGT               A^CGCGT
MluNI         130  TGGCCA               TGG^CCA
MroI          668  TCCGGA               T^CCGGA
MscI          130  TGGCCA               TGG^CCA
MslI         1044  CATCGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PstI          393  CTGCAG               CTGCA^G
PvuII         126  CAGCTG               CAG^CTG
RcaI          225  TCATGA               T^CATGA
SacII         142  CCGCGG               CCGC^GG
SfcI          393  CTGCAG               C^T[GA][CT]AG
SfiI          131  GGCCAGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI          249  CCAAGG               C^C[AT][AT]GG
TfiI         1458  GATTC                G^A[AT]TC
Tsp45I        479  GTGAC                ^GT[GC]AC
Tsp509I       180  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

Aor51HI       506  AGCGCT               AGC^GCT
             1034  AGCGCT              
BsiWI         806  CGTACG               C^GTACG
              890  CGTACG              
BspMI        1451  ACCTGC               ACCTGC(4/8)
              154  GCAGGT              
BssSI          75  CACGAG               CACGAG(-5/-1)
              694  CACGAG              
BstAPI        899  GCAAGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1067  GCAGCGAGTGC         
EaeI          130  TGGCCA               [CT]^GGCC[GA]
             1320  CGGCCA              
Eco47III      506  AGCGCT               AGC^GCT
             1034  AGCGCT              
PpuMI          69  GGGACCC              [GA]G^G[AT]CC[CT]
               93  GGGTCCT             
SacI          927  GAGCTC               GAGCT^C
             1011  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
Alw44I      AlwI        ApaLI       Asp700I     AspHI       
AvaII       BamHI       BanII       BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrFI       BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeII       
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SgrAI       
StuI        TaqI        TseI        TspRI       TthHB8I     
XhoII       XmnI