Restriction map for
pF1KB9643
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 126 GCGG CCGC(-3/-1)
AcyI 455 GGCGTC G[GA]^CG[CT]C
AfaI 1177 GTAC GT^AC
AflIII 375 ACATGT A^C[GA][CT]GT
AhdI 450 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 196 ATTAAT AT^TAAT
AsnI 196 ATTAAT AT^TAAT
AspEI 450 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 364 GAGCAC G[AT]GC[AT]^C
AvaII 991 GGTCC G^G[AT]CC
BbvCI 706 GCTGAGG CCTCAGC(-5/-2)
BciVI 483 GGATAC GTATCC(6/5)
BmyI 364 GAGCAC G[AGT]GC[ACT]^C
BpmI 933 CTGGAG CTGGAG(16/14)
BsaBI 252 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 455 GGCGTC G[GA]^CG[CT]C
BsiHKAI 364 GAGCAC G[AT]GC[AT]^C
BsmFI 429 GTCCC GGGAC(10/14)
Bsp1286I 364 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 375 ACATGT A^CATGT
BspMI 207 GCAGGT ACCTGC(4/8)
BsrDI 702 CATTGC GCAATG(2/0)
BssSI 356 CTCGTG CACGAG(-5/-1)
Cfr13I 991 GGTCC G^G[ACGT]CC
DsaI 58 CCACGG C^C[GA][CT]GG
Eam1105I 450 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 433 CTCTTC CTCTTC(1/4)
EcoRI 129 GAATTC G^AATTC
EcoT14I 488 CCTTGG C^C[AT][AT]GG
HgaI 456 GCGTC GACGC(5/10)
Hin1I 455 GGCGTC G[GA]^CG[CT]C
HphI 1122 GGTGA GGTGA(8/7)
Ksp632I 433 CTCTTC CTCTTC(1/4)
MamI 252 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 152 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 331 CAGCTG C[AC]G^C[GT]G
NdeI 641 CATATG CA^TATG
PflMI 848 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 427 GAGTC GAGTC(4/5)
PshBI 196 ATTAAT AT^TAAT
PstI 205 CTGCAG CTGCA^G
PvuII 331 CAGCTG CAG^CTG
RsaI 1177 GTAC GT^AC
Sau96I 991 GGTCC G^G[ACGT]CC
SbfI 204 CCTGCAGG CCTGCA^GG
SmlI 899 CTCAAG C^T[CT][GA]AG
Sse8387I 204 CCTGCAGG CCTGCA^GG
StyI 488 CCTTGG C^C[AT][AT]GG
TaqI 875 TCGA T^CGA
Tsp45I 1123 GTGAC ^GT[GC]AC
TthHB8I 875 TCGA T^CGA
Van91I 848 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 185 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
517 CAGTTACTG
BbsI 859 GAAGAC GAAGAC(2/6)
379 GTCTTC
BglII 18 AGATCT A^GATCT
257 AGATCT
BpuAI 859 GAAGAC GAAGAC(2/6)
379 GTCTTC
BsiYI 452 CCTGGCGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
848 CCAGTCAGTGG
BslI 452 CCTGGCGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
848 CCAGTCAGTGG
BstAPI 89 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
747 GCAACAGATGC
BstF5I 417 GGATG GGATG(2/0)
689 GGATG
FokI 417 GGATG GGATG(9/13)
689 GGATG
HaeIII 693 GGCC GG^CC
973 GGCC
MwoI 89 GCAAAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
747 GCAACAGATGC
SfcI 205 CTGCAG C^T[GA][CT]AG
514 CTACAG
SpeI 496 ACTAGT A^CTAGT
870 ACTAGT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BsaI BsaJI BsmAI BsrI
BssKI BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NspI Sau3AI ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI XhoII