Restriction map for
pF1KB9642
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Aor51HI 563 AGCGCT AGC^GCT
ApaI 665 GGGCCC GGGCC^C
BamHI 1559 GGATCC G^GATCC
BbsI 379 GTCTTC GAAGAC(2/6)
BcgI 345 GCAGGGCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 885 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 885 GCTCAGC GC^T[ACGT]AGC
BpuAI 379 GTCTTC GAAGAC(2/6)
BsaI 1316 GAGACC GGTCTC(1/5)
BsmBI 604 CGTCTC CGTCTC(1/5)
BsmI 233 GAATGC GAATGC(1/-1)
BspMI 1225 ACCTGC ACCTGC(4/8)
BstAPI 228 GCAGGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1598 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 885 GCTCAGC GC^T[ACGT]AGC
CfoI 564 GCGC GCG^C
DrdI 1042 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 563 AGCGCT AGC^GCT
HaeII 563 AGCGCT [GA]GCGC^[CT]
HhaI 564 GCGC GCG^C
HinP1I 564 GCGC G^CGC
NheI 534 GCTAGC G^CTAGC
PstI 343 CTGCAG CTGCA^G
SexAI 500 ACCTGGT A^CC[AT]GGT
SfaNI 4 GATGC GCATC(5/9)
SfcI 343 CTGCAG C^T[GA][CT]AG
SfiI 32 GGCCCCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 615 ACTAGT A^CTAGT
TaqI 1260 TCGA T^CGA
Tsp509I 263 AATT ^AATT
TthHB8I 1260 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 160 GTAC GT^AC
816 GTAC
AlwNI 475 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
581 CAGTGCCTG
BanI 798 GGTGCC G^G[CT][GA]CC
1719 GGCACC
BglI 33 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1209 GCCTCCAGGGC
BsgI 108 CTGCAC GTGCAG(16/14)
1420 GTGCAG
BstEII 1050 GGTCACC G^GT[ACGT]ACC
1753 GGTAACC
BstPI 1050 GGTCACC G^GT[ACGT]ACC
1753 GGTAACC
EcoNI 252 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1337 CCTGGATGAGG
EcoO65I 1050 GGTCACC G^GT[ACGT]ACC
1753 GGTAACC
MspA1I 87 CAGCTG C[AC]G^C[GT]G
659 CAGCTG
PleI 170 GACTC GAGTC(4/5)
1477 GACTC
PvuII 87 CAGCTG CAG^CTG
659 CAGCTG
RsaI 160 GTAC GT^AC
816 GTAC
SacI 187 GAGCTC GAGCT^C
792 GAGCTC
SapI 1179 GAAGAGC GCTCTTC(1/4)
1773 GCTCTTC
SphI 928 GCATGC GCATG^C
1287 GCATGC
StuI 387 AGGCCT AGG^CCT
1345 AGGCCT
TfiI 362 GAATC G^A[AT]TC
855 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwI AocI AspHI
AvaII BalI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PpuMI Sau3AI Sau96I ScrFI
SmlI StyI TseI Tsp45I TspRI
Van91I XcmI XhoII