Restriction map for
pF1KB9611
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI KspI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 139 GACGTC GACGT^C
AcsI 1504 GAATTC [GA]^AATT[CT]
AhdI 1369 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 520 GTGCAC G^TGCAC
ApaLI 520 GTGCAC G^TGCAC
ApoI 1504 GAATTC [GA]^AATT[CT]
AspEI 1369 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 253 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1137 TGGCCA TGG^CCA
BamHI 824 GGATCC G^GATCC
BanI 1113 GGCGCC G^G[CT][GA]CC
BbeI 1113 GGCGCC GGCGC^C
BbvCI 1004 CCTCAGC CCTCAGC(-5/-2)
BfaI 200 CTAG C^TAG
BsaBI 1479 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 277 CTGCAC GTGCAG(16/14)
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BsrDI 1304 GCAATG GCAATG(2/0)
BsrFI 485 GCCGGC [GA]^CCGG[CT]
BstEII 846 GGTCACC G^GT[ACGT]ACC
BstPI 846 GGTCACC G^GT[ACGT]ACC
BstXI 440 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 485 GCCGGC [GA]^CCGG[CT]
DraIII 388 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 21 GACCCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1369 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 64 CTCTTC CTCTTC(1/4)
EcoO65I 846 GGTCACC G^GT[ACGT]ACC
EcoRI 1504 GAATTC G^AATTC
HincII 259 GTCAAC GT[CT]^[GA]AC
HindII 259 GTCAAC GT[CT]^[GA]AC
HindIII 787 AAGCTT A^AGCTT
KasI 1113 GGCGCC G^GCGCC
Ksp632I 64 CTCTTC CTCTTC(1/4)
MaeI 200 CTAG C^TAG
MamI 1479 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1137 TGGCCA TGG^CCA
MscI 1137 TGGCCA TGG^CCA
MslI 1704 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 485 GCCGGC GCC^GGC
NarI 1113 GGCGCC GG^CGCC
NgoMIV 485 GCCGGC G^CCGGC
PaeR7I 591 CTCGAG C^TCGAG
PflFI 253 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1642 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 1010 CGATCG CGAT^CG
PvuII 1148 CAGCTG CAG^CTG
SacI 691 GAGCTC GAGCT^C
SbfI 735 CCTGCAGG CCTGCA^GG
SexAI 932 ACCAGGT A^CC[AT]GGT
SfoI 1113 GGCGCC GGC^GCC
SmlI 591 CTCGAG C^T[CT][GA]AG
Sse8387I 735 CCTGCAGG CCTGCA^GG
StuI 74 AGGCCT AGG^CCT
Tsp509I 1505 AATT ^AATT
Tth111I 253 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 785 CCAAGCTTATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 591 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 825 GATCC GGATC(4/5)
824 GGATC
Asp700I 426 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
990 GAAGGAGTTC
AspHI 520 GTGCAC G[AT]GC[AT]^C
691 GAGCTC
AvaI 591 CTCGAG C^[CT]CG[GA]G
688 CCCGAG
BanII 691 GAGCTC G[GA]GC[CT]^C
1044 GGGCTC
BbsI 628 GTCTTC GAAGAC(2/6)
1204 GTCTTC
BglI 184 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1513 GCCGCCTCGGC
BglII 926 AGATCT A^GATCT
1478 AGATCT
BlpI 1145 GCTCAGC GC^T[ACGT]AGC
1150 GCTGAGC
Bpu1102I 1145 GCTCAGC GC^T[ACGT]AGC
1150 GCTGAGC
BpuAI 628 GTCTTC GAAGAC(2/6)
1204 GTCTTC
BsiEI 159 CGACCG CG[GA][CT]^CG
1010 CGATCG
BsiHKAI 520 GTGCAC G[AT]GC[AT]^C
691 GAGCTC
BsmBI 747 CGTCTC CGTCTC(1/5)
529 GAGACG
BsoBI 591 CTCGAG C^[CT]CG[GA]G
688 CCCGAG
BstAPI 119 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1583 GCATGTCCTGC
CelII 1145 GCTCAGC GC^T[ACGT]AGC
1150 GCTGAGC
EcoNI 561 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
735 CCTGCAGGAGG
HaeII 1000 AGCGCC [GA]GCGC^[CT]
1113 GGCGCC
HinfI 352 GAGTC G^A[ACGT]TC
1659 GACTC
MaeII 56 ACGT A^CGT
140 ACGT
MspA1I 1148 CAGCTG C[AC]G^C[GT]G
1457 CCGCTG
NcoI 228 CCATGG C^CATGG
1140 CCATGG
PleI 1659 GACTC GAGTC(4/5)
352 GAGTC
TaqI 592 TCGA T^CGA
632 TCGA
TthHB8I 592 TCGA T^CGA
632 TCGA
XmnI 426 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
990 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI Van91I XhoII