Restriction map for
pF1KB9594
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbsI BbvCI BcgI BciVI
BclI BfaI BlnI BpmI BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI Eam1105I EarI Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 334 CTTAAG C^TTAAG
Alw44I 970 GTGCAC G^TGCAC
AlwI 219 GATCC GGATC(4/5)
ApaLI 970 GTGCAC G^TGCAC
BanII 81 GAGCTC G[GA]GC[CT]^C
BbrPI 649 CACGTG CAC^GTG
BfrI 334 CTTAAG C^TTAAG
BglII 113 AGATCT A^GATCT
BsgI 265 CTGCAC GTGCAG(16/14)
BsmFI 281 GGGAC GGGAC(10/14)
BspLU11I 284 ACATGT A^CATGT
BsrBI 595 CCGCTC CCGCTC(-3/-3)
BsrI 606 CCAGT ACTGG(1/-1)
BssHII 413 GCGCGC G^CGCGC
BstXI 689 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 958 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 143 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 370 GACGC GACGC(5/10)
HincII 609 GTTGAC GT[CT]^[GA]AC
HindII 609 GTTGAC GT[CT]^[GA]AC
HinfI 676 GACTC G^A[ACGT]TC
HphI 935 TCACC GGTGA(8/7)
KspI 223 CCGCGG CCGC^GG
MboII 93 GAAGA GAAGA(8/7)
NspI 284 ACATGT [GA]CATG^[CT]
PleI 676 GACTC GAGTC(4/5)
PmaCI 649 CACGTG CAC^GTG
PmlI 649 CACGTG CAC^GTG
PstI 385 CTGCAG CTGCA^G
SacI 81 GAGCTC GAGCT^C
SacII 223 CCGCGG CCGC^GG
SmlI 334 CTTAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 311 GGCGCC G[GA]^CG[CT]C
351 GGCGCC
AlwNI 252 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
379 CAGTACCTG
Aor51HI 164 AGCGCT AGC^GCT
320 AGCGCT
AspHI 81 GAGCTC G[AT]GC[AT]^C
970 GTGCAC
AvaII 132 GGACC G^G[AT]CC
234 GGACC
BalI 71 TGGCCA TGG^CCA
643 TGGCCA
BbeI 311 GGCGCC GGCGC^C
351 GGCGCC
BcnI 10 CCCGG CC^[GC]GG
942 CCCGG
BglI 367 GCCGACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
546 GCCTGGGGGGC
BsaAI 21 CACGTA [CT]AC^GT[GA]
649 CACGTG
BsaHI 311 GGCGCC G[GA]^CG[CT]C
351 GGCGCC
BsiHKAI 81 GAGCTC G[AT]GC[AT]^C
970 GTGCAC
BspMI 383 ACCTGC ACCTGC(4/8)
437 ACCTGC
BstAPI 378 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
573 GCAGCCGGTGC
BstYI 113 AGATCT [GA]^GATC[CT]
218 AGATCC
Eco47III 164 AGCGCT AGC^GCT
320 AGCGCT
EcoT14I 617 CCATGG C^C[AT][AT]GG
695 CCATGG
Hin1I 311 GGCGCC G[GA]^CG[CT]C
351 GGCGCC
KasI 311 GGCGCC G^GCGCC
351 GGCGCC
MflI 113 AGATCT [GA]^GATC[CT]
218 AGATCC
MluNI 71 TGGCCA TGG^CCA
643 TGGCCA
MscI 71 TGGCCA TGG^CCA
643 TGGCCA
MseI 335 TTAA T^TAA
788 TTAA
NaeI 664 GCCGGC GCC^GGC
798 GCCGGC
NarI 311 GGCGCC GG^CGCC
351 GGCGCC
NciI 10 CCCGG CC^[GC]GG
942 CCCGG
NcoI 617 CCATGG C^CATGG
695 CCATGG
NgoMIV 664 GCCGGC G^CCGGC
798 GCCGGC
SfoI 311 GGCGCC GGC^GCC
351 GGCGCC
StyI 617 CCATGG C^C[AT][AT]GG
695 CCATGG
Tru9I 335 TTAA T^TAA
788 TTAA
XhoII 113 AGATCT [GA]^GATC[CT]
218 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AvaI BanI BbvI BlpI BmyI
Bpu1102I BsaJI BsiEI BsiYI BslI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TthHB8I