Restriction map for
pF1KB6256
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 1227 GACGTC GACGT^C
AccII 1521 CGCG CG^CG
AcsI 1432 GAATTT [GA]^AATT[CT]
AfaI 1524 GTAC GT^AC
ApaI 1515 GGGCCC GGGCC^C
ApoI 1432 GAATTT [GA]^AATT[CT]
AvaII 1010 GGACC G^G[AT]CC
BbsI 956 GTCTTC GAAGAC(2/6)
BcgI 155 GCAAGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1644 GTATCC GTATCC(6/5)
BclI 1160 TGATCA T^GATCA
BglI 598 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 956 GTCTTC GAAGAC(2/6)
BsmI 51 GAATGC GAATGC(1/-1)
BspMI 1747 GCAGGT ACCTGC(4/8)
BsrDI 941 GCAATG GCAATG(2/0)
BssSI 1990 CACGAG CACGAG(-5/-1)
BstUI 1521 CGCG CG^CG
DraI 1447 TTTAAA TTT^AAA
DsaI 2000 CCACGG C^C[GA][CT]GG
FbaI 1160 TGATCA T^GATCA
HindIII 1442 AAGCTT A^AGCTT
MaeII 1228 ACGT A^CGT
MvnI 1521 CGCG CG^CG
NheI 1606 GCTAGC G^CTAGC
RsaI 1524 GTAC GT^AC
SapI 159 GCTCTTC GCTCTTC(1/4)
SpeI 978 ACTAGT A^CTAGT
StuI 932 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AspHI 780 GAGCTC G[AT]GC[AT]^C
912 GAGCTC
BcnI 307 CCGGG CC^[GC]GG
1198 CCCGG
BsgI 1264 CTGCAC GTGCAG(16/14)
1078 GTGCAG
BsiHKAI 780 GAGCTC G[AT]GC[AT]^C
912 GAGCTC
BsmAI 810 GTCTC GTCTC(1/5)
1293 GTCTC
BsrBI 835 CCGCTC CCGCTC(-3/-3)
1711 GAGCGG
EcoNI 1027 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1575 CCTACCACAGG
HincII 573 GTCAAC GT[CT]^[GA]AC
974 GTCAAC
HindII 573 GTCAAC GT[CT]^[GA]AC
974 GTCAAC
NaeI 295 GCCGGC GCC^GGC
1995 GCCGGC
NciI 307 CCGGG CC^[GC]GG
1198 CCCGG
NgoMIV 295 GCCGGC G^CCGGC
1995 GCCGGC
PflMI 423 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
814 CCAGGACATGG
SacI 780 GAGCTC GAGCT^C
912 GAGCTC
SmlI 1708 CTTGAG C^T[CT][GA]AG
1831 CTTGAG
TaqI 1042 TCGA T^CGA
1743 TCGA
TfiI 127 GAATC G^A[AT]TC
408 GAATC
Tsp45I 967 GTCAC ^GT[GC]AC
1185 GTCAC
TthHB8I 1042 TCGA T^CGA
1743 TCGA
Van91I 423 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
814 CCAGGACATGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
BanI BanII BbeI BbvCI BbvI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NarI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI Tru9I
TseI Tsp509I TspRI XhoII