Restriction map for
pF1KB6259
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanII BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HpaI KpnI KspI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SbfI SfiI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 292 GTCGAC GT^[AC][GT]AC
AcyI 1296 GGCGCC G[GA]^CG[CT]C
AgeI 2084 ACCGGT A^CCGGT
Aor51HI 839 AGCGCT AGC^GCT
Asp700I 1605 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1212 GAGCAC G[AT]GC[AT]^C
AvaI 1450 CTCGGG C^[CT]CG[GA]G
BalI 32 TGGCCA TGG^CCA
BbeI 1296 GGCGCC GGCGC^C
BpmI 1191 CTCCAG CTGGAG(16/14)
BsaAI 2252 TACGTA [CT]AC^GT[GA]
BsaHI 1296 GGCGCC G[GA]^CG[CT]C
BsaWI 2084 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1212 GAGCAC G[AT]GC[AT]^C
BsmAI 1000 GAGAC GTCTC(1/5)
BsmI 1349 GAATGC GAATGC(1/-1)
BsoBI 1450 CTCGGG C^[CT]CG[GA]G
BspHI 1418 TCATGA T^CATGA
BstBI 1694 TTCGAA TT^CGAA
BstXI 1436 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 696 GACAGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 377 CCGTGG C^C[GA][CT]GG
EarI 1816 GAAGAG CTCTTC(1/4)
Eco47III 839 AGCGCT AGC^GCT
EcoT14I 1130 CCTTGG C^C[AT][AT]GG
EcoT22I 320 ATGCAT ATGCA^T
HgaI 1076 GCGTC GACGC(5/10)
Hin1I 1296 GGCGCC G[GA]^CG[CT]C
KasI 1296 GGCGCC G^GCGCC
Ksp632I 1816 GAAGAG CTCTTC(1/4)
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
NarI 1296 GGCGCC GG^CGCC
NsiI 320 ATGCAT ATGCA^T
NspV 1694 TTCGAA TT^CGAA
PinAI 2084 ACCGGT A^CCGGT
PstI 1741 CTGCAG CTGCA^G
RcaI 1418 TCATGA T^CATGA
SalI 292 GTCGAC G^TCGAC
SapI 1816 GAAGAGC GCTCTTC(1/4)
SexAI 782 ACCTGGT A^CC[AT]GGT
SfcI 1741 CTGCAG C^T[GA][CT]AG
SfoI 1296 GGCGCC GGC^GCC
SfuI 1694 TTCGAA TT^CGAA
SnaBI 2252 TACGTA TAC^GTA
StuI 1458 AGGCCT AGG^CCT
StyI 1130 CCTTGG C^C[AT][AT]GG
XcmI 567 CCAGTTGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1605 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BbsI 86 GAAGAC GAAGAC(2/6)
765 GAAGAC
BciVI 814 GTATCC GTATCC(6/5)
907 GTATCC
BmyI 862 GTGCCC G[AGT]GC[ACT]^C
1212 GAGCAC
BpuAI 86 GAAGAC GAAGAC(2/6)
765 GAAGAC
BsaBI 1108 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1616 GATGTCCATC
Bsp1286I 862 GTGCCC G[AGT]GC[ACT]^C
1212 GAGCAC
BspDI 1669 ATCGAT AT^CGAT
2004 ATCGAT
BspMI 1871 ACCTGC ACCTGC(4/8)
901 GCAGGT
BsrDI 455 GCAATG GCAATG(2/0)
1039 GCAATG
BsrFI 200 ACCGGC [GA]^CCGG[CT]
2084 ACCGGT
BstAPI 1039 GCAATGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1749 GCAAGGCTTGC
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
2084 ACCGGT
ClaI 1669 ATCGAT AT^CGAT
2004 ATCGAT
DraI 925 TTTAAA TTT^AAA
937 TTTAAA
EaeI 32 TGGCCA [CT]^GGCC[GA]
257 TGGCCG
EcoRI 877 GAATTC G^AATTC
1697 GAATTC
HaeII 839 AGCGCT [GA]GCGC^[CT]
1296 GGCGCC
HapII 201 CCGG C^CGG
2085 CCGG
HindIII 1718 AAGCTT A^AGCTT
2077 AAGCTT
HpaII 201 CCGG C^CGG
2085 CCGG
MaeII 995 ACGT A^CGT
2253 ACGT
MamI 1108 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1616 GATGTCCATC
MspI 201 CCGG C^CGG
2085 CCGG
PflMI 304 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1102 CCACTTGATGG
PleI 1448 GACTC GAGTC(4/5)
619 GAGTC
PpuMI 1504 AGGACCC [GA]G^G[AT]CC[CT]
1919 AGGTCCC
Van91I 304 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1102 CCACTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BfaI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I