Restriction map for
pF1KB9979
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
Ksp632I KspI MluI MluNI MroI
MscI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 540 GGCGTC G[GA]^CG[CT]C
AfaI 1590 GTAC GT^AC
Alw44I 899 GTGCAC G^TGCAC
ApaI 920 GGGCCC GGGCC^C
ApaLI 899 GTGCAC G^TGCAC
BbrPI 109 CACGTG CAC^GTG
BciVI 338 GGATAC GTATCC(6/5)
BclI 1457 TGATCA T^GATCA
BcnI 873 CCGGG CC^[GC]GG
BfaI 1622 CTAG C^TAG
BglII 18 AGATCT A^GATCT
BsaAI 109 CACGTG [CT]AC^GT[GA]
BsaBI 430 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 540 GGCGTC G[GA]^CG[CT]C
BsgI 1445 CTGCAC GTGCAG(16/14)
BsiEI 1508 CGATCG CG[GA][CT]^CG
BsmI 817 GAATGC GAATGC(1/-1)
Bsp1407I 1589 TGTACA T^GTACA
BspMI 1397 ACCTGC ACCTGC(4/8)
BsrGI 1589 TGTACA T^GTACA
BstXI 1143 CCAGACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 18 AGATCT [GA]^GATC[CT]
DrdI 1146 GACTCATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 594 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 309 GAATTC G^AATTC
EcoRV 1072 GATATC GAT^ATC
EcoT14I 1046 CCATGG C^C[AT][AT]GG
FbaI 1457 TGATCA T^GATCA
HapII 873 CCGG C^CGG
Hin1I 540 GGCGTC G[GA]^CG[CT]C
HpaII 873 CCGG C^CGG
MaeI 1622 CTAG C^TAG
MaeIII 549 GTCAC ^GT[ACGT]AC
MamI 430 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 197 CAATTG C^AATTG
MflI 18 AGATCT [GA]^GATC[CT]
MspI 873 CCGG C^CGG
MunI 197 CAATTG C^AATTG
NciI 873 CCGGG CC^[GC]GG
NcoI 1046 CCATGG C^CATGG
NdeI 245 CATATG CA^TATG
PflMI 1353 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1146 GACTC GAGTC(4/5)
PmaCI 109 CACGTG CAC^GTG
PmlI 109 CACGTG CAC^GTG
PstI 1399 CTGCAG CTGCA^G
PvuI 1508 CGATCG CGAT^CG
RsaI 1590 GTAC GT^AC
SspBI 1589 TGTACA T^GTACA
StyI 1046 CCATGG C^C[AT][AT]GG
TaqI 815 TCGA T^CGA
Tsp45I 549 GTCAC ^GT[GC]AC
TthHB8I 815 TCGA T^CGA
Van91I 1353 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 18 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 999 GCGG CCGC(-3/-1)
1160 GCGG
AcsI 263 AAATTC [GA]^AATT[CT]
309 GAATTC
AlwI 66 GGATC GGATC(4/5)
812 GGATC
AlwNI 1096 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1393 CAGGACCTG
AocI 132 CCTAAGG CC^T[ACGT]AGG
631 CCTCAGG
ApoI 263 AAATTC [GA]^AATT[CT]
309 GAATTC
BanI 734 GGCACC G^G[CT][GA]CC
1463 GGCACC
BbsI 1138 GAAGAC GAAGAC(2/6)
104 GTCTTC
BpuAI 1138 GAAGAC GAAGAC(2/6)
104 GTCTTC
BseRI 607 CTCCTC GAGGAG(10/8)
1471 CTCCTC
BsrDI 757 GCAATG GCAATG(2/0)
1363 GCAATG
BssSI 551 CACGAG CACGAG(-5/-1)
1558 CACGAG
Bsu36I 132 CCTAAGG CC^T[ACGT]AGG
631 CCTCAGG
DsaI 1046 CCATGG C^C[GA][CT]GG
1256 CCACGG
Eco81I 132 CCTAAGG CC^T[ACGT]AGG
631 CCTCAGG
HgaI 472 GCGTC GACGC(5/10)
541 GCGTC
HincII 203 GTCAAC GT[CT]^[GA]AC
586 GTTGAC
HindII 203 GTCAAC GT[CT]^[GA]AC
586 GTTGAC
HindIII 52 AAGCTT A^AGCTT
218 AAGCTT
HphI 1335 GGTGA GGTGA(8/7)
1526 TCACC
MaeII 110 ACGT A^CGT
624 ACGT
PshAI 97 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1323 GACTATGGTC
SacI 1439 GAGCTC GAGCT^C
1645 GAGCTC
SfaNI 45 GATGC GCATC(5/9)
765 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AspHI AvaI AvaII BanII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PpuMI PvuII Sau3AI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI