Restriction map for
pF1KE0692
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# NO CUT ENZYMES ( 143 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BsaAI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tsp45I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 276 GACGTC GACGT^C
AccI 595 GTCTAC GT^[AC][GT]AC
AccII 22 CGCG CG^CG
Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
AspHI 447 GTGCAC G[AT]GC[AT]^C
AspI 589 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 588 GGACC G^G[AT]CC
BbeI 61 GGCGCC GGCGC^C
BfaI 1195 CTAG C^TAG
BlpI 397 GCTAAGC GC^T[ACGT]AGC
BmyI 447 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 397 GCTAAGC GC^T[ACGT]AGC
BsaBI 429 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 601 GAGACC GGTCTC(1/5)
BsiHKAI 447 GTGCAC G[AT]GC[AT]^C
Bsp1286I 447 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 878 TGTACA T^GTACA
BsrDI 567 CATTGC GCAATG(2/0)
BsrGI 878 TGTACA T^GTACA
BsrI 962 ACTGG ACTGG(1/-1)
BstAPI 402 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 22 CGCG CG^CG
CelII 397 GCTAAGC GC^T[ACGT]AGC
DraIII 220 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 259 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 47 CCAAGG C^C[AT][AT]GG
HaeII 61 GGCGCC [GA]GCGC^[CT]
HapII 653 CCGG C^CGG
HpaII 653 CCGG C^CGG
HphI 670 TCACC GGTGA(8/7)
KasI 61 GGCGCC G^GCGCC
MaeI 1195 CTAG C^TAG
MaeII 277 ACGT A^CGT
MaeIII 506 GTTAC ^GT[ACGT]AC
MamI 429 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 551 TTAA T^TAA
MspA1I 469 CAGCGG C[AC]G^C[GT]G
MspI 653 CCGG C^CGG
MvnI 22 CGCG CG^CG
NarI 61 GGCGCC GG^CGCC
PflFI 589 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 778 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 510 CTACAG C^T[GA][CT]AG
SfoI 61 GGCGCC GGC^GCC
SspBI 878 TGTACA T^GTACA
StuI 1173 AGGCCT AGG^CCT
StyI 47 CCAAGG C^C[AT][AT]GG
Tru9I 551 TTAA T^TAA
Tth111I 589 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 778 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 47 CCAAGGAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 883 AAATTT [GA]^AATT[CT]
977 AAATTC
AcyI 61 GGCGCC G[GA]^CG[CT]C
276 GACGTC
AocI 709 CCTGAGG CC^T[ACGT]AGG
744 CCTAAGG
ApoI 883 AAATTT [GA]^AATT[CT]
977 AAATTC
BbsI 199 GAAGAC GAAGAC(2/6)
958 GAAGAC
BglII 693 AGATCT A^GATCT
1109 AGATCT
BpuAI 199 GAAGAC GAAGAC(2/6)
958 GAAGAC
BsaHI 61 GGCGCC G[GA]^CG[CT]C
276 GACGTC
BspHI 330 TCATGA T^CATGA
531 TCATGA
Bsu36I 709 CCTGAGG CC^T[ACGT]AGG
744 CCTAAGG
Cac8I 213 GCAAGC GC[ACGT]^[ACGT]GC
482 GCAAGC
CfoI 62 GCGC GCG^C
411 GCGC
Eco81I 709 CCTGAGG CC^T[ACGT]AGG
744 CCTAAGG
HhaI 62 GCGC GCG^C
411 GCGC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
276 GACGTC
HinP1I 62 GCGC G^CGC
411 GCGC
MslI 304 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
378 CATGATAGTG
PleI 667 GACTC GAGTC(4/5)
1151 GAGTC
RcaI 330 TCATGA T^CATGA
531 TCATGA
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BpmI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HinfI ItaI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp509I TspRI XhoII