Restriction map for
pF1KB7690
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 411 CGCG CG^CG
AhdI 640 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 730 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 640 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 135 GAGCAC G[AT]GC[AT]^C
AvaI 132 CCCGAG C^[CT]CG[GA]G
BanI 633 GGTGCC G^G[CT][GA]CC
BciVI 79 GGATAC GTATCC(6/5)
BcnI 147 CCGGG CC^[GC]GG
BglII 315 AGATCT A^GATCT
BlpI 931 GCTGAGC GC^T[ACGT]AGC
BmyI 135 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 931 GCTGAGC GC^T[ACGT]AGC
BsaJI 67 CCAGGG C^C[ACGT][ACGT]GG
BsgI 443 GTGCAG GTGCAG(16/14)
BsiHKAI 135 GAGCAC G[AT]GC[AT]^C
BsmFI 242 GGGAC GGGAC(10/14)
BsoBI 132 CCCGAG C^[CT]CG[GA]G
Bsp1286I 135 GAGCAC G[AGT]GC[ACT]^C
BstAPI 445 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 158 CATCC GGATG(2/0)
BstUI 411 CGCG CG^CG
BstXI 1059 CCAACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 931 GCTGAGC GC^T[ACGT]AGC
CfoI 936 GCGC GCG^C
DraI 345 TTTAAA TTT^AAA
DraII 674 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 640 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 765 GAAGAG CTCTTC(1/4)
EcoNI 215 CCTACATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 674 AGGTCCT [GA]G^G[ACGT]CC[CT]
FokI 158 CATCC GGATG(9/13)
HapII 147 CCGG C^CGG
HhaI 936 GCGC GCG^C
HinP1I 936 GCGC G^CGC
HpaII 147 CCGG C^CGG
HphI 1069 GGTGA GGTGA(8/7)
Ksp632I 765 GAAGAG CTCTTC(1/4)
MslI 1011 CATTCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 147 CCGG C^CGG
MvnI 411 CGCG CG^CG
NciI 147 CCGGG CC^[GC]GG
NspI 305 GCATGT [GA]CATG^[CT]
PpuMI 674 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 857 CAGCTG CAG^CTG
SfcI 114 CTACAG C^T[GA][CT]AG
SspI 341 AATATT AAT^ATT
StuI 392 AGGCCT AGG^CCT
XmnI 730 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 614 AAATTC [GA]^AATT[CT]
772 AAATTC
AlwI 532 GATCC GGATC(4/5)
1204 GATCC
AlwNI 38 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
446 CAGAAGCTG
AocI 958 CCTGAGG CC^T[ACGT]AGG
1090 CCTGAGG
ApoI 614 AAATTC [GA]^AATT[CT]
772 AAATTC
BfaI 665 CTAG C^TAG
721 CTAG
BsmI 668 GAATGC GAATGC(1/-1)
515 GCATTC
BspMI 672 GCAGGT ACCTGC(4/8)
687 GCAGGT
Bsu36I 958 CCTGAGG CC^T[ACGT]AGG
1090 CCTGAGG
Eco81I 958 CCTGAGG CC^T[ACGT]AGG
1090 CCTGAGG
FauI 409 CCCGC CCCGC(4/6)
887 CCCGC
HincII 470 GTCGAC GT[CT]^[GA]AC
1120 GTCGAC
HindII 470 GTCGAC GT[CT]^[GA]AC
1120 GTCGAC
MaeI 665 CTAG C^TAG
721 CTAG
MaeII 641 ACGT A^CGT
1081 ACGT
MspA1I 205 CCGCTG C[AC]G^C[GT]G
857 CAGCTG
SalI 470 GTCGAC G^TCGAC
1120 GTCGAC
TaqI 471 TCGA T^CGA
1121 TCGA
Tsp45I 151 GTCAC ^GT[GC]AC
655 GTCAC
TthHB8I 471 TCGA T^CGA
1121 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccI AciI AfaI AluI AvaII
BbvI BseRI BsiYI BslI BsmAI
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HinfI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI XhoII