Restriction map for
pF1KB9967
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AccIII AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaAI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 364 GACGTC GACGT^C
Acc65I 1251 GGTACC G^GTACC
AccI 367 GTCTAC GT^[AC][GT]AC
AclI 511 AACGTT AA^CGTT
Alw44I 705 GTGCAC G^TGCAC
AlwI 1307 GGATC GGATC(4/5)
ApaI 43 GGGCCC GGGCC^C
ApaLI 705 GTGCAC G^TGCAC
Asp718I 1251 GGTACC G^GTACC
AvaI 283 CTCGGG C^[CT]CG[GA]G
BbsI 1336 GTCTTC GAAGAC(2/6)
BcgI 141 GCACCGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 649 CTAG C^TAG
BglI 338 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1336 GTCTTC GAAGAC(2/6)
BsaBI 1497 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 219 CGGCCG CG[GA][CT]^CG
BsoBI 283 CTCGGG C^[CT]CG[GA]G
Bsp1407I 534 TGTACA T^GTACA
BsrDI 1419 CATTGC GCAATG(2/0)
BsrFI 251 ACCGGC [GA]^CCGG[CT]
BsrGI 534 TGTACA T^GTACA
Cfr10I 251 ACCGGC [GA]^CCGG[CT]
DrdI 1023 GACCATTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 219 CGGCCG [CT]^GGCC[GA]
EagI 219 CGGCCG C^GGCCG
EclXI 219 CGGCCG C^GGCCG
Eco52I 219 CGGCCG C^GGCCG
HgaI 160 GACGC GACGC(5/10)
HindIII 977 AAGCTT A^AGCTT
KpnI 1251 GGTACC GGTAC^C
MaeI 649 CTAG C^TAG
MamI 1497 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 527 CCATGG C^CATGG
PflMI 569 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 130 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 511 AACGTT AA^CGTT
SacI 1348 GAGCTC GAGCT^C
ScaI 1209 AGTACT AGT^ACT
SspBI 534 TGTACA T^GTACA
SspI 1009 AATATT AAT^ATT
TaqI 150 TCGA T^CGA
TthHB8I 150 TCGA T^CGA
Van91I 569 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
AlwNI 177 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
759 CAGAGACTG
BanII 43 GGGCCC G[GA]GC[CT]^C
1348 GAGCTC
BglII 620 AGATCT A^GATCT
1585 AGATCT
BlpI 72 GCTCAGC GC^T[ACGT]AGC
1345 GCTGAGC
Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC
1345 GCTGAGC
BsaHI 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
BsmI 1457 GAATGC GAATGC(1/-1)
1648 GCATTC
BstXI 626 CCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCATTGGTCTGG
BstYI 620 AGATCT [GA]^GATC[CT]
1585 AGATCT
CelII 72 GCTCAGC GC^T[ACGT]AGC
1345 GCTGAGC
DsaI 527 CCATGG C^C[GA][CT]GG
1394 CCACGG
EarI 1237 CTCTTC CTCTTC(1/4)
1541 CTCTTC
EcoRV 852 GATATC GAT^ATC
883 GATATC
EcoT14I 527 CCATGG C^C[AT][AT]GG
1631 CCAAGG
Hin1I 160 GACGCC G[GA]^CG[CT]C
364 GACGTC
Ksp632I 1237 CTCTTC CTCTTC(1/4)
1541 CTCTTC
MflI 620 AGATCT [GA]^GATC[CT]
1585 AGATCT
StyI 527 CCATGG C^C[AT][AT]GG
1631 CCAAGG
Tsp45I 385 GTGAC ^GT[GC]AC
1387 GTCAC
XhoII 620 AGATCT [GA]^GATC[CT]
1585 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I AspHI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp509I TspRI
XmnI