Restriction map for
pF1KB7644
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvrII BamHI BbeI BbvCI BcgI
BclI BfrI BglII BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRV FbaI FseI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I MamI MfeI MflI
MluI MroI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 24 CGCG CG^CG
AfaI 601 GTAC GT^AC
Aor51HI 248 AGCGCT AGC^GCT
AspHI 729 GAGCTC G[AT]GC[AT]^C
AspI 319 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1023 TGCGCA TGC^GCA
BanI 662 GGCACC G^G[CT][GA]CC
BbrPI 553 CACGTG CAC^GTG
BbsI 424 GAAGAC GAAGAC(2/6)
BciVI 284 GTATCC GTATCC(6/5)
BglI 543 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 424 GAAGAC GAAGAC(2/6)
BsaAI 553 CACGTG [CT]AC^GT[GA]
BseRI 696 GAGGAG GAGGAG(10/8)
BsiHKAI 729 GAGCTC G[AT]GC[AT]^C
BsmI 519 GAATGC GAATGC(1/-1)
BspMI 332 ACCTGC ACCTGC(4/8)
BsrDI 1020 CATTGC GCAATG(2/0)
BsrFI 362 ACCGGC [GA]^CCGG[CT]
BstUI 24 CGCG CG^CG
Cfr10I 362 ACCGGC [GA]^CCGG[CT]
DrdI 589 GACATGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 248 AGCGCT AGC^GCT
EcoRI 385 GAATTC G^AATTC
EcoT22I 521 ATGCAT ATGCA^T
FauI 22 CCCGC CCCGC(4/6)
FspI 1023 TGCGCA TGC^GCA
HaeII 248 AGCGCT [GA]GCGC^[CT]
KspI 23 CCGCGG CCGC^GG
MvnI 24 CGCG CG^CG
NsiI 521 ATGCAT ATGCA^T
NspI 1026 GCATGC [GA]CATG^[CT]
PflFI 319 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 454 GACTC GAGTC(4/5)
PmaCI 553 CACGTG CAC^GTG
PmlI 553 CACGTG CAC^GTG
PstI 734 CTGCAG CTGCA^G
RsaI 601 GTAC GT^AC
SacI 729 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
SfcI 734 CTGCAG C^T[GA][CT]AG
SphI 1026 GCATGC GCATG^C
StuI 582 AGGCCT AGG^CCT
TaqI 1145 TCGA T^CGA
Tsp45I 755 GTGAC ^GT[GC]AC
Tth111I 319 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1145 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 223 GTCTAC GT^[AC][GT]AC
325 GTCTAC
AcsI 385 GAATTC [GA]^AATT[CT]
785 AAATTC
AlwI 780 GGATC GGATC(4/5)
1155 GGATC
ApoI 385 GAATTC [GA]^AATT[CT]
785 AAATTC
BalI 371 TGGCCA TGG^CCA
801 TGGCCA
BfaI 58 CTAG C^TAG
1127 CTAG
BlpI 469 GCTAAGC GC^T[ACGT]AGC
765 GCTGAGC
Bpu1102I 469 GCTAAGC GC^T[ACGT]AGC
765 GCTGAGC
BsgI 334 CTGCAC GTGCAG(16/14)
170 GTGCAG
CelII 469 GCTAAGC GC^T[ACGT]AGC
765 GCTGAGC
DpnII 781 GATC ^GATC
1156 GATC
DraII 131 AGGTCCT [GA]G^G[ACGT]CC[CT]
1071 GGGACCC
EaeI 371 TGGCCA [CT]^GGCC[GA]
801 TGGCCA
EcoNI 393 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
642 CCTGAAGAAGG
EcoO109I 131 AGGTCCT [GA]G^G[ACGT]CC[CT]
1071 GGGACCC
EcoT14I 872 CCATGG C^C[AT][AT]GG
1061 CCATGG
MaeI 58 CTAG C^TAG
1127 CTAG
MaeIII 755 GTGAC ^GT[ACGT]AC
838 GTTAC
MboI 781 GATC ^GATC
1156 GATC
MluNI 371 TGGCCA TGG^CCA
801 TGGCCA
MscI 371 TGGCCA TGG^CCA
801 TGGCCA
MseI 514 TTAA T^TAA
853 TTAA
NcoI 872 CCATGG C^CATGG
1061 CCATGG
NdeII 781 GATC ^GATC
1156 GATC
PpuMI 131 AGGTCCT [GA]G^G[AT]CC[CT]
1071 GGGACCC
Sau3AI 781 GATC ^GATC
1156 GATC
SmlI 163 CTCAAG C^T[CT][GA]AG
903 CTTGAG
StyI 872 CCATGG C^C[AT][AT]GG
1061 CCATGG
TfiI 78 GATTC G^A[AT]TC
926 GAATC
Tru9I 514 TTAA T^TAA
853 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DsaI EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MaeII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PvuII Sau96I ScrFI
SfaNI TseI Tsp509I TspRI