Restriction map for
pF1KB5768
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MfeI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1968 CGCG CG^CG
AciI 670 GCGG CCGC(-3/-1)
AflIII 1967 ACGCGT A^C[GA][CT]GT
Alw44I 2113 GTGCAC G^TGCAC
AlwI 532 GATCC GGATC(4/5)
ApaLI 2113 GTGCAC G^TGCAC
BanI 1340 GGTGCC G^G[CT][GA]CC
BbrPI 888 CACGTG CAC^GTG
BbvI 1506 GCTGC GCAGC(8/12)
BclI 1443 TGATCA T^GATCA
BsaAI 888 CACGTG [CT]AC^GT[GA]
BsaBI 2011 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 294 GAATGC GAATGC(1/-1)
BspDI 799 ATCGAT AT^CGAT
BspHI 1428 TCATGA T^CATGA
BstAPI 2131 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1968 CGCG CG^CG
BstYI 531 AGATCC [GA]^GATC[CT]
ClaI 799 ATCGAT AT^CGAT
DraI 141 TTTAAA TTT^AAA
DraII 825 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 825 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2034 GAATTC G^AATTC
EcoRV 1671 GATATC GAT^ATC
FbaI 1443 TGATCA T^GATCA
Fnu4HI 1506 GCTGC GC^[ACGT]GC
HapII 2210 CCGG C^CGG
HindIII 358 AAGCTT A^AGCTT
HpaII 2210 CCGG C^CGG
ItaI 1506 GCTGC GC^[ACGT]GC
MamI 2011 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 531 AGATCC [GA]^GATC[CT]
MluI 1967 ACGCGT A^CGCGT
MspI 2210 CCGG C^CGG
MvnI 1968 CGCG CG^CG
PflMI 757 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 932 GAGTC GAGTC(4/5)
PmaCI 888 CACGTG CAC^GTG
PmlI 888 CACGTG CAC^GTG
PpuMI 825 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1428 TCATGA T^CATGA
TseI 1506 GCTGC G^C[AT]GC
Van91I 757 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 339 TCTAGA T^CTAGA
XcmI 1945 CCAGTCTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 531 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 1975 GTCTAC GT^[AC][GT]AC
2074 GTAGAC
Asp700I 149 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
196 GAATATCTTC
BciVI 1879 GGATAC GTATCC(6/5)
1599 GTATCC
BsaI 641 GGTCTC GGTCTC(1/5)
1996 GGTCTC
BseRI 1574 CTCCTC GAGGAG(10/8)
1750 CTCCTC
BsmFI 827 GTCCC GGGAC(10/14)
1628 GTCCC
Cac8I 48 GCTTGC GC[ACGT]^[ACGT]GC
615 GCTTGC
DsaI 2091 CCACGG C^C[GA][CT]GG
2199 CCACGG
EarI 560 GAAGAG CTCTTC(1/4)
604 GAAGAG
EcoT14I 1709 CCTTGG C^C[AT][AT]GG
1737 CCAAGG
HgaI 1436 GACGC GACGC(5/10)
1969 GCGTC
Ksp632I 560 GAAGAG CTCTTC(1/4)
604 GAAGAG
MaeII 889 ACGT A^CGT
1883 ACGT
MslI 1821 CATAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2235 CACCCACATG
PstI 212 CTGCAG CTGCA^G
1632 CTGCAG
ScaI 228 AGTACT AGT^ACT
1379 AGTACT
SmlI 861 CTCAAG C^T[CT][GA]AG
2020 CTTGAG
StyI 1709 CCTTGG C^C[AT][AT]GG
1737 CCAAGG
XmnI 149 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
196 GAATATCTTC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BbsI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I